Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
I have prepared agarose gel, now i want to dissolve the gel completely in order to do the further analysis, I have put in the beaker and added some water to the gel and then I tried stirring at...
18 June 2020 3,954 10 View
I wonder if anybody has an experience of sequencing PCR products where master mix contains green dye for gel loading. What if I will purify them with enzymes instead of gel extraction or...
19 May 2020 5,956 5 View
Hi, guys, I have a queation, please help me. I used air liquid interface method to do 3D culture. This is the first time to do it. the collagen gel prepared according to literature, 8 vol of...
14 May 2020 7,397 2 View
Hello everyone, In order to perform cell cycle analysis, I need to harvest cells from 6wp. I wonder what is the optimal volume of trypsin I should add to each well. I will appreciate any help in...
05 May 2020 6,182 0 View
hello, I have 3 questions- 1.In all publications i see that they used TBE buffer in EMSA (polyacrylamide gel), why? can I try in the binding reaction and running the gel with other buffers?...
02 May 2020 9,258 0 View
I am trying to prepare gel based hand sanitizer using WHO formulation with IPA. Water based hand rub is 80% alcohol, when I am replacing water by CMC gel solution of 2% it is not dissolving in...
30 April 2020 2,851 3 View
Please find attached a photo of the problem; After a simple Agarose gel electrophoresis of DNA with EtBr i find the gel "divided" in two parts. Analizing at the gel at 259nm it seems that the...
29 April 2020 2,475 7 View
Till now I've used SIFT, PROVEAN, POLYPHEN-2. What other servers are out their that analysis the deleterious effects of SNPs and their impact?
11 April 2020 1,797 5 View
In the place where I work we manufacture our own GelMa. However, lately we have been having some problems, so we need to characterize the degree of methacrylation of the Gel. Do you have any...
02 April 2020 362 2 View
Hi researcher and fellows, the protein bands separated differently as compared in two gels (12.5%). The sample for both is the same but it looks like weird in the second gel. That gel did after I...
29 March 2020 7,889 4 View
Ethosomes suspension, Ethosomes gel, Ethosomes containing transdermal patches
25 March 2020 5,729 1 View
I extracted DNA from several bacterial isolates and I amplified the V1-V3 region of the 16S rRNA gene of these samples. However, when I ran a DGGE with these samples, several lanes did not contain...
24 March 2020 2,964 8 View
How much percentage of gel used for puc18 vector for agarose gel electrophoresis
18 March 2020 293 3 View
I have been working on making Polyacrylamide gels from 30% Acrylamide and 2% bisacryalmide stock solutions. I prepare the polymer solutions in a centrifuge rube, add APS and TEMED to the solution,...
18 March 2020 5,296 3 View
Hi, I am working with polysaccharides from plant samples. One of the purification steps is to add ethanol to the water extract to precipitate polysaccharides. However, in some cases, the...
18 March 2020 8,439 3 View
I have a liquid polyanhydride that is not soluble in water made from succinic acid and ricinoleic acid and I need to make it a hard gel. I think I can cross-link using ethylenediamine, but I'm...
17 March 2020 4,361 4 View
Hi everyone ! Any tips and tricks to section 3D culture/ stem cell spheroid of 1K cells density using Histo gel or Agar gel ?
15 March 2020 2,515 3 View
I have TPTA gel of various concentration and want to get an idea of the pore size distribution and its crosslinking density.
13 March 2020 3,295 3 View
Dear all, recently i have tried to prepare a composite gel containing gelatin with transglutaminase. After placing the mix inside the incubator, the entire gel turned white. Why is this...
12 March 2020 2,938 4 View
Dear all, I am culturing human primary keratinocytes and fibroblast together to make an organotypic model of skin. I already had the following collagen solution in my lab so I used...
11 March 2020 4,320 3 View
So, I'm formulating a microemulgel. the appearence of my microemulsion and gel base was clear and transparant. but when i combine the two phases, the microemulgel becomes cloudy. what could...
11 March 2020 6,613 4 View
If we used carbopol for gel formation and after adding the other excipients and neutralizing of the polymer solution, some fine white particles like granules or very small flaked were observed...
11 March 2020 6,343 3 View
Hello you all, I would like to compare a protein sequence between different organism in order to check whether it is a conservative protein or not. I have used blastp to check that sequence...
11 March 2020 4,131 3 View
I am trying to detect ATM (a very high protein) by Western Blot; I have transferred overnight at 4ºC and at 40mA but I have not been successful. Any suggestion??
06 March 2020 8,060 1 View