Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
This thought experiment has been bugging me for quite some time, and I’d love to hear perspectives from the community. Setup: Imagine two parallel class-one levers (Levers 1 and 2 in the figure),...
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There are many methods to extract DNA from MRSA, but which one is the best?
30 July 2025 3,642 1 View
I am currently working on total RNA extraction from Fragaria × ananassa (strawberry) using the CTAB method. As part of the protocol, I need to prepare a Lithium Chloride (LiCl) solution, typically...
20 July 2025 1,456 2 View
Hi all, I am trying to identify a receptor in primary immune cells for my protein of interest, but I am not seeing any potential bands in the elution buffer. For the immunoprecipitation (IP), I...
08 July 2025 7,834 3 View
Would greatly appreciate any insight as to whether it is possible to use a Microelectrode Puller (specifically, the PUL-1000 y WPI) to produce needles with a total shaft length of less than 50 mm....
04 July 2025 1,333 0 View
I am currently working on RNA extraction from mango peel tissues (both raw and ripened stages) for gene expression analysis. However, I’m facing a persistent issue across different protocols and...
03 June 2025 2,174 2 View
Hi everyone, I’m trying to simulate liquefaction using PM4Sand in FLAC8.1. For now, I only simulate the ground (no structure), and I use a sinusoidal wave as the input motion. The pore pressure...
01 June 2025 5,100 1 View
Some patients present with back pain due to breast ca metastasis on MRI. MRI shows whole spine metastasis, without evidence of fractures. Is their any indication for vertebroplasty as pain relief...
14 May 2025 2,613 1 View
I am currently culturing SH-SY5Y, and overall, the cells appear healthy and are proliferating well. However, I have consistently observed some extra structures or growths around the cells under...
25 April 2025 7,392 7 View
So I am working with SH-SY5Y cell line and when I seed my cells they're exhibiting uniform growth, however on the day of treatment, when I discard the media from the 96-well plate, I can't see the...
07 April 2025 7,907 5 View
In summary, during the expression process, my protein underwent significant translational interruption, leading to the production of a large amount of truncated target protein. I heterologously...
17 January 2025 9,429 3 View
how much exosome and dye are need? incubation time? and centrifuge?
16 January 2025 2,306 2 View
I am currently conducting a protein overexpression experiment and encountered an issue. I amplified the cDNA of my target protein using primers with an HA-tag sequence (attached to the 5’ end) via...
24 December 2024 1,713 2 View
How to add error bar in the XRD, UV and other studies?. Kindly give a suggestion
11 November 2024 9,516 5 View
Repeat domain of tau protein is often called either K18 (4 repeats) or K19 (3 repeats). Why are these called K something fragment? Where did the name come from? What does the K stand for? I...
07 November 2024 1,512 1 View
Hi, Research Community I am working on a two-step enzymatic reaction, wherein 1st step requires Mn2+ while 2nd step needs Mg2+ supplementation for optimum cleavage of substrates. The presence of...
01 October 2024 7,807 3 View
How to prepare 5% Binder solution of cellulose acetate ?
10 September 2024 273 2 View
I'm looking for protocols/sources that provide information about how to maximize the isolation of membrane bound proteins and general protein yield of unstimulated primary CD4+ T-cells. I have...
26 August 2024 4,304 3 View
Green House Gas Mitigation and Carnon Sequestration is very important when we consider Soil Micoflora? Let us try to find different answers which should be helpful for the researchers and friends...
06 August 2024 4,479 4 View
if yes, is the publication free of charge
07 June 2024 4,311 1 View
Hello! I am trying to stain primary endothelial cells that have been grown on Matrigel to form tubules. I am interested in apical-basal polarity. The cells do form tubules but it is hard to image...
29 May 2024 5,901 4 View
Does lateral shift produce increases with increase in the refractive index of the medium and relationship between lateral displacement and refractive index?
19 May 2024 7,997 0 View
Hello! I want to try Npas1 staining on post mortem FFPE brain tissue, but I have no previous experience with Npas1 and am not sure which antibody to start with. If anybody has good experience with...
26 April 2024 6,715 0 View
Hello! Any idea of which housekeeping gene has more stable expression in A431 and SCC-13 cell lines (squamous cell carcinoma), in order to use it as reference gene in my qPCR? Didn't have any lack...
15 April 2024 480 2 View