Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
Give me strengths and weakness of this research paper
02 August 2023 9,216 0 View
In my new lab, we assess RNA quality using formaldehyde. I have four different RNA concentrations: 405, 232.3, 1216.7, and 3772. To standardize the concentrations, I normalized them and then...
31 July 2023 7,676 3 View
Hi everyone! Currently im working in Zymography for amylase from seed sample, i was doing based on this reference with slightly modification Amylolytic activities and starch reserve mobilization...
19 July 2023 4,516 0 View
I am running RT-qPCR for a gene. However I still see amplification with lower Cq values in my negative control. Previously the Cq values of the negative controls were above 30. But now it averages...
04 July 2023 7,182 4 View
I need doxorubicin resistant breast cancer cells (MDA-MB-231, 4T1 preference). It will take several months to develop the resistant strains. I will be grateful for ever if any of you is willing...
27 June 2023 5,995 1 View
I performed zymogram analysis for lipase using this protocol: 1. Run Native -PAGE until adequate migration (roughly 80V for top gel: 30min, 120V for bottom gel: 2 hour) at 4 °C. Cathode connects...
06 June 2023 6,596 5 View
I have tried with ethidium bromide and stains-all for staining but not getting any band after one day of incubation. I tried with both Native and Non-Native gel.
06 June 2023 336 3 View
I have been trying to visualize supercomplexes in BN PAGE, however my results are inconsistent. All the single complexes are visible, but supercomplexes are not. I am using an imidazole based...
02 June 2023 650 1 View
I have a fluorescent sample and want to measure the excitation and emission wavelength using the Synergy HT BioTek microplate reader. I have tried spectrophotometer ND1000 and 3300, but want to...
31 May 2023 3,792 1 View
We have linked aminobenzoic acid dissolved in PBS 1x with SPDP (succinimidyl 3-(2-pyridyldithio)propionate) dissovled first in a small volume of DMSO (3% of the total reacton volume). We want to...
07 May 2023 7,788 1 View
Dear Friends, I have a problem with an #inverted #microscope lens, therefore if you can recommend an application to improve the pictures, please tell me.
06 May 2023 7,208 2 View
Hello I ran a Gradient-PCR, annealing Temp ranging from 63 to 67. I am getting a faint band at the top of the gel and down to my GOI band. (Image attached). I have tried nearly everything from...
02 May 2023 7,456 2 View
In order to prepare the stock solution for enzyme assay, I need to verify that my NADPH salt is not oxidized. What precautions should I take during the preparation of the stock solution?
30 April 2023 2,328 0 View
I have a chemical reaction which was dissolved in DMF. How can I perform TLC for that reaction before I proceed with the workup and evaporate the DMF?
06 April 2023 5,446 2 View
Hi all, I am using hyaluronidase from sheep testes for breakdown of hyaluronic acid and analysing by HPLC. Is it possible for hyaluronidase to perform more "destruction" to hyaluronic acid of...
05 April 2023 1,456 0 View
I have genome data of yeast and want to find out it's ITS region. Can anyone suggest any tool or method to find out the ITS region?
03 April 2023 6,665 2 View
To whom should I ask permission if I want to use a figure from a paper published in ResearchGate? Do I need to ask permission from ResearchGate or the Author?
28 March 2023 7,886 1 View
I am working on some Gastric cancer cell lines, and i want to use lentiviral based crispr cas9 technology to knockout a gene from GC cell lines, previously the problem was mainly in the poor...
23 March 2023 4,855 4 View
Seeking expert advice from academia. Both my bachelor’s and master’s are in the field of computer science. I have research, industry, and entrepreneurship experiences related to that field...
16 March 2023 8,753 3 View
I have performed venom fractionation using HPLC and run the samples in SDS PAGE. I wanted to know what could be the possible reason for my protein bands to look like this. It should have been...
16 March 2023 3,485 6 View
I'm preparing for two protein immunoblot assay from cell lysate. The reference what i referred used two lysis buffer for cell lysis. For immunoprecipitation before western blot, NETN buffer...
13 March 2023 9,634 1 View
Is it possible to fabricate "experimentally" graphene multi-layers with a thickness of 30 nm? What is the limitation of this thickness?
12 March 2023 3,676 6 View
I'm trying to detect intracellular iron levels in my cells in-vitro and stained using prussian blue. I fixed the cells in 4% paraformaldehyde, washed and let them sit in prussian blue mix from...
09 March 2023 3,688 1 View
Facts: Amplified ribosomal DNA restriction analysis (ARDRA) involves the amplification of the 16S rRNA gene (1500 bp) followed by enzymatic restriction and gel electrophoresis. The terminal...
03 March 2023 2,916 1 View