Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
Should the final gel needs visible or UV light to stimulate the TiO2 nanoparticles to work as antibacterial?
23 July 2021 6,251 6 View
Do methanol fixation and paraformaldehyde alter the ability to detect the foci of 53BP1 in cells? Also, I would be interested to know if the protocol of pre-extraction (using the solution -25 mM...
17 July 2021 479 0 View
Could you recommend some supporting resources to refer to this matter. Thanks!
04 July 2021 6,846 10 View
Hi all, I am thinking about other approaches than SEC to separate protein-dye conjugates from free fluorescent dyes, like Alexa, Cy and Atto dyes. Does any of you have experience with that? Will...
18 June 2021 9,225 7 View
How to prepare Ethidium Bromide, What concentration of EtBr is required, and what is the correct amount to be added to the gel?
27 May 2021 662 3 View
Hello, I'm currently doing my thesis (for my bachelor's degree) for industrial engineering, where the objective is to achieve hydrogels to remove metal ions from the acid mine drainage based on...
10 May 2021 5,384 0 View
if I have the following data: slope:- 5.34066 Intercept :- 0.959409 correlation coefficient, r :- 0.994797
03 May 2021 9,664 2 View
There is a backlog for deionized formamide from all the suppliers in Canada. Do you know what I can use instead of deionized formamide for conducting the whole-mount in situ hybridization in...
03 May 2021 5,245 1 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
24 February 2021 8,486 2 View
Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...
17 February 2021 2,016 1 View
We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...
16 February 2021 4,479 4 View
Hi good day to everyone! I am actually studying the gel content of synthetic latex. Refer to ASTM 3616, gel content is measure of insoluble rubbers in the chosen solvent. So, I just would like to...
08 February 2021 868 4 View
I came to know about removal of SDS from Polyacrylamide gel is done by Triton-X, but what is the exact mechanism of its removal. How Triton-x does that? Thank you
04 February 2021 7,348 2 View
Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....
03 February 2021 5,140 2 View
Our lab discovered a gel that was left overnight on our alphaimager with the UV light left on. It is now rock solid and very thin, fused to the surface of the imager.
02 February 2021 6,890 2 View
When we cast a 10% SDS PAGE gel, the protein resolution is not sufficient and is similar to an 8% gel. We tried using a freshly prepared acrylamide from a different box, but the problem still...
31 January 2021 999 2 View
One of my project requirements is to measure temperature and humidity in the growing environment of plant. The plant will be grown inside a petri dish with nutrient gel. A waterproof temperature...
29 January 2021 9,537 2 View
So I did the BCA assay and came up with these concentrations/ul: Sample 1 = 2.9 ug/ul, Sample 2 = 2.5 ug/ul, Sample 3 = 3.5 ug/ul, Sample 4 = 2.7 ug/ul. I want to calculate how much of each to...
25 January 2021 5,419 4 View
I am willing to Cas13 in vitro. Can it be directly purified from PAGE gel, please suggest me the method.
20 January 2021 1,305 3 View
I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end...
18 January 2021 4,307 1 View
Hi. I am trying to study a biotin modification of my target protein in a crude cell extract and am interested to determine how much of my protein in getting biotinylated. After biotinylating my...
16 January 2021 2,338 4 View
Currently have punched mouse brain samples of different regions that are of too low protein concentration for Western Blot. Has anyone used the polyacrylamide gel electrophoresis method to detect...
10 January 2021 4,083 3 View
I am doing an adhesion experiment on cancer cells. In various previous literature, researchers used different substrates (Glass, TCPS, Hydrogels). Sometimes they do the coating on the substrates...
04 January 2021 2,070 0 View
I am working on the capillary formation of Lymphatic endothelial cells in a collagen matrix. But I am facing a technical problem to handle Collagen. I have made many times; I am not able to remove...
28 December 2020 5,590 3 View