Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
I'm trying to detect intracellular iron levels in my cells in-vitro and stained using prussian blue. I fixed the cells in 4% paraformaldehyde, washed and let them sit in prussian blue mix from...
09 March 2023 3,703 1 View
Facts: Amplified ribosomal DNA restriction analysis (ARDRA) involves the amplification of the 16S rRNA gene (1500 bp) followed by enzymatic restriction and gel electrophoresis. The terminal...
03 March 2023 2,932 1 View
Hey friends, a week ago, I received a notification from the ResearchGate team that they will be retiring projects by 31st march. Has anyone of you received a such a notification? What should I o...
01 March 2023 5,973 4 View
When I casting Urea PAGE gel, I can see the gel shrink in bottom and no wells formed but gel solidified. Does anyone know a possible reason or how to minimize this?
23 February 2023 5,660 5 View
Dear Researchers, I have tried to dissolve CeO2 powder in to acetone using sol-gel method. However, the powder got precipitated and not dissolving. So give your suggestion to get solution for the...
18 February 2023 2,652 1 View
I dissolved C16-H33-KTTKS (pal-KTTKS) in acetic acid, methanol, and other solvents and analyzed them with HPLC, but they did not detect. Could you please assist me in dissolving it while it is...
15 February 2023 3,217 0 View
I'm looking for the doubling time of the BDCM cell line, I didn't find any information about it
12 February 2023 2,335 1 View
So I created what I thought was a chitosan hydrogel, but some of my lab mates were saying it was a sponge. How do I know for sure whether it is a hydrogel or a sponge?
12 February 2023 9,994 3 View
Hello everyone. I have a question about VIP score. I am currently working on microbiota data, and I am using the mixOmics package on R to get VIP scores so that I can filter the most "important"...
10 February 2023 6,550 1 View
I have read many protocols for the preparation of cross-linked hyaluronic acid gel for soft tissue fillers, but cross-linking active ingredients are hard to find in my country, so I am looking for...
06 February 2023 3,931 0 View
We want to make an array of interdigitated electrodes (IDEs), to make an array of sensor. What is a suitable design for it? How IDEs should be connected to each other? How to take connections or...
01 February 2023 6,004 0 View
I'm trying to carry out metadynamics for a system, where my collective variable is bond length. Is there way to decide how to set values for height and width ? I tried with two different values...
30 January 2023 6,349 3 View
Hello! My skeletal muscle biology lab investigates the effect Notch signaling has on muscle repair. We have had issues with the housekeeping protein signal (Beta-actin) being too irregular. I...
22 January 2023 9,827 0 View
Hello: my name is Ricki and I am a PhD student from the University of Southern California (USC). I have a question about CdSe quantum dots I purchased from Ocean Nanotech...
21 January 2023 3,933 0 View
Hello all, I have been working with the DIFI cancer cell line and upon staining with Hoescht (cyan) and EdU (green, S phase marker), I noticed these circular structures in the cytoplasm. My...
17 January 2023 7,782 4 View
I am trying to purify a protein that is expressed in NEB10 beta E.coli competent cells and has his-tag. I used Ni-NTA column however Ni-NTA did not fully purify and I had activity in the flow...
12 January 2023 9,889 1 View
Hi, During RNA isolation, why is it required to spin the tubes not more than 10000 rpm rather than 12000 rpm after adding "isopropanol"? Thanks!
11 January 2023 832 6 View
Hello all! I am performing a plaque assay for a phage I isolated from a sewage sample and was wondering if it's normal that lower dilutions show colonies on a fairly clear base (from my...
30 December 2022 9,369 1 View
I'm trying to think of a way to over-express RNA using a plasmid. Previously, we have clone a RNA of interest (e.g., lncRNA) into pcDNA3.1/Zeo (Thermo Fisher Scientific, #V860–20). Because there...
29 December 2022 6,137 6 View
Can anybody please share the Glycerol and Sucrose estimation protocol? I want to check the Glycerol and Sucrose concentration in the yeast culture. I really appreciate any help you can provide.
27 December 2022 7,942 4 View
I did several times gel agarose electrophoresis to find the best N/P ratio of chitosan/tRNA polyplex. I don't know why I could not observe the tRNA in the well of gel agarose electrophoresis and...
17 December 2022 7,199 8 View
It will be helpful if anyone suggests places to perform the CMC analysis.
08 December 2022 722 1 View
Hi, I work in research right now but I used to work in the medical field, and when I created standard dilutions for viral assay controls I would make a concoctions using I believe 10mM Tris-HCL,...
07 December 2022 9,703 1 View
I have a technical problem with SDS-PAGE, the running sample is a protein extract dissolved in 100 mM NaCl solution, and others dissolved in 10mM PBS solution pH 8.0, the protein concentration per...
07 December 2022 2,829 9 View