Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
Is it necessary to carry out validation tests on model mixtures prepared using both substances, or is it enough to use one substance for testing? What validation tests should be carried out for...
05 December 2022 1,160 8 View
I've noticed while precipitant in 10X and 1X TBST buffers in my lab. I can't quite say for sure whether they're the same precipitant or different types. I would like to ask whether they might be...
03 December 2022 5,844 4 View
This is a small question with big implications for quantity calculations. If I purify a peptide with lysines or other compounds with free amines with preparative HPLC using 0.1% formic acid and...
30 November 2022 1,073 5 View
I need the list of protein to do docking study on anticancer activity.
29 November 2022 2,195 2 View
Good day! I'm using the BioRad PROTEAN II XI chamber and gradiend polyakrylamide gel - 3.3%-20%. I used to run the gel overnight at 105-110 V, but this time got the "Error no load" after ~6 hours...
24 November 2022 8,605 4 View
Hi, I am looking for a fragment analyzer for QC of RNA and DNA. According to Bioptic web (https://www.bioptic.com.tw/applications/laboratory-solution/high-resolution), their instrument can resolve...
23 November 2022 2,172 0 View
Isolating DNA from plasma using a column. DNA concentration on nanodrop is 5ng/ul. Unable to see any band in gel after 3-5 ul load no result in PCR amplification? what can be the reason?
18 November 2022 6,530 8 View
Hi, I would like to express a toxic construct in HeLa and 293T cells. It has to be easily inducible and absolutely not leaky. I did some research and only few promoters are available: Tet...
11 November 2022 6,648 0 View
Hi all, Recently I am working on protein purification. 1. affinity column(GST) 2. Add PSP enzyme to remove GST-tag(O/N) 3. GST-tag off 4. gel filtration However, the protein tends to form large...
08 November 2022 9,412 2 View
Hi, I'm working with human genomic DNA and try to analyse SNPs after PCR and digestion. If i get now 3 bands after PCR and restiction enzyme incubation for partly digested it could mean 2...
07 November 2022 6,252 5 View
Purified preps of IgG (via Protein G column) have yielded multiple bands under reducing conditions (mercaptoethanol, 5 mins, 95c) on SDS PAGE. Could you please help me identify the bands by...
05 November 2022 3,226 3 View
ddsolver is add-in software in excel used for dissolution studies
03 November 2022 8,256 0 View
Just recently, I succeeded in making gold nanoparticles. Please assist with the preparation of gold nanoparticles for SEM and TEM.
31 October 2022 3,018 5 View
I am using 8% gel and Bio-Rad Precision Plus Protein Dual Color Standards. I have been loading 15ul of my ladder, and when I decreased the loaded amount to 5ul, I got the same weird appearance.
26 October 2022 7,633 3 View
Hello all! I have completed a test yeast expression for 5 different proteins, and I have taken a sample of the culture at 0, 24, 48 and 72 hours. 3 of my yeast cultures seemingly expressed a...
12 October 2022 3,318 5 View
Hello, In qPCR having a set of primers and a Taqman probe, how to determine the size of the amplicon (to be) without running a gel from the PCR product? Would the presence/position of a hydrolysis...
10 October 2022 3,749 2 View
We have two quant studio 3 real time pcr machines, no service contracts. One of the machines can run an experiment and the touch screen does work; however, none of the touch screen features work....
16 September 2022 6,566 0 View
Hi! I want to measure the sugar concentration of gummy bear. Is it possible to do by mixing the gummy with 100 ml distilled water and measure the concentration of solution over time until it reach...
06 September 2022 2,264 3 View
I ran my RNA samples in bleach gel with dsDNA ladder on the same gel (Bleach gel: a simple agarose gel for analyzing RNA quality - PubMed (nih.gov)). However, my DNA ladder, even triple amount...
19 August 2022 4,495 0 View
Does anyone have experience and willing to share their knowledge on liquid micro junction-surface sampling probes? What are the best adaptation of instruments for a available LC/MS? For example,...
05 August 2022 3,582 0 View
Good day! How we are having a project, in which one of the stages consist of evaluation of the structure of hemicellulose polysaccharides. However, not so many literature is dedicated to such a...
26 June 2022 2,722 0 View
Any lab uses a specific breeding scheme (Tg male or female to breed with nonTg parent) to generate Thy1-APPPS1 (Jucker) mice heterozygous pups to investigate amyloidosis? Please let me know!
18 June 2022 2,280 1 View
after pouring and drying when i am going to take out my sample then its harder whats the reason?
07 June 2022 7,405 3 View
Hello, I would like help in detecting and marking in western blotting. We in our laboratory do not have a chemiluminescence detection system. Would it be possible to detect chemiluminescence using...
03 June 2022 1,933 0 View