Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
1,276 views 1,541 posts
Questions related to Gels
Hello everyone, I want to find out how the co-culture of two anaerobic bacteria influences each other in order to find competition and cross-feeding relations between them. My first approach is...
15 October 2020 316 1 View
Hi ! I am starting my PhD studying the interractions between macrophages and the tumoral extracellular matrix and I am trying to design an experiment to assess the effect of the stiffness of the...
12 October 2020 923 1 View
I am doing an electrophoresis using a Tris-Acetate gel and the vendor offers a 1.0 mm and 1.5 mm thick gels. I just want some insight on the advantages. Thank you
08 October 2020 3,378 2 View
Would some one propose an effective method to measure the diffusion coefficient of a transparent solution in a transparent gel (agarose)? Thank you in advance.
06 October 2020 3,003 3 View
Hi, everyone! I am really confused. From yesterday, my stack gel of BN-PAGE never polymerize. However, the same materials I used for making the running gels, which polymerize normally. I was...
03 October 2020 3,352 1 View
Both my marker and protein are stacking but they are not going through the stacking gel into the separating gel. Please help. My stacking gel concentration is 4% while the separating gel...
28 September 2020 8,226 2 View
Hello and good morning to all of you, hope you all will be fine, i have a question regarding gel electrophorosis, i was using SDS-PAG for phloem sap proteins, but i achieved the bands even lower...
14 September 2020 3,654 2 View
Hi everyone, In order to do CHIP experiment, I used some lysis buffer and micrococcus followed by sonication to digest chromatin of the cells. I did all the process of cross-lincking, quenching,...
12 September 2020 1,433 3 View
i am Iqra, i am doing research on purification of amylase i want to perform zymogram of amylase. anyone send me the protocol for native PAGE electrophoresis.
08 September 2020 4,898 3 View
I am looking forward to evaluating changes in the proteome level of mouse brain after drug treatment. I have done two trial experiments of MS-MS run with in-gel tryptic digestion and I also...
08 September 2020 235 3 View
Hi. I've recently ran few protein samples alongside with pre-stained ladder on a SDS-PAGE. Everything looks fine on the gel. Then, I did the transfer onto PVDF membrane for protein detection. All...
07 September 2020 7,846 3 View
When post-staining agarose gels in an ethidium bromide / water bath for 10 minutes, my labmate says she can re-use the post-stain solution 2-3 times before she has to add more ethidium bromide (at...
05 September 2020 905 3 View
Hello, I have isolated a phage from a soil sample. I got a LTL somewhere around 10^9 pfu/mL. I did DNA extractions and got great DNA concentrations, always over 100ng/uL and the 260/280 ratio...
05 September 2020 6,029 3 View
In other words, given the gel percentage, voltage, protein size, buffer concentration, can you predict how fast a protein or DNA segment should theoretically travel down the gel? A related...
01 September 2020 4,142 1 View
This picture the product from my ligation and transformation. Eventhough, it is from the same host but I get different size and I wonder which one I should use to send for sequencing. And what the...
17 August 2020 9,285 3 View
I am using TBE-Urea gels in PAGE to analyze ssDNA. About half of the protocols (like Qiagen and Biorad) out there insist on pre-running the gel at constant wattage to pre-heat it. However, others...
05 August 2020 4,797 2 View
Dear researchers, I would like to ask is it necessary to do Elution of proteins from Polyacrylamide gel before sending the protein sample to Nano-LC-TOF-MS Analysis? This is what I plan, I would...
02 August 2020 3,819 3 View
I want to do MMP digestion on my collagen gels to see the effect of glycation on my collagen gels. I have difficulty to find the best MMP! some articles used MMP-1 some MMP-9 some MMP -2 and some...
29 July 2020 6,820 1 View
Patient diagnosed with intractable major depression. Olanzapine treatment caused severe drug-induced Parkinsonian symptoms. Olanzapine discontinued 18 months ago and patient recovered from DIP....
16 July 2020 1,869 7 View
Hello, I'm recently facing with serious problems with my Western Blot experiments. I'm now doing this protocol since 4 years (same cells, same antibodies, same amount of protein loaded etc...) and...
10 July 2020 5,310 6 View
I have to score the gel images generated by ISSR markers. Could you please explain how scoring the gel images for ISSR markers?
09 July 2020 6,003 2 View
Want to set up a 2D protein gel system. Can anybody recommend a system to go for? We use a lot of Bio-Rad's protein gel systems, but don't know which one is best for 2D gels.
08 July 2020 6,436 3 View
I am studying the re-swelling kinetics of dried hydrogel films. I want to use a rheometer to follow the modulus decrease during the re-swelling. However, during the re-swelling both the thickness...
02 July 2020 1,123 5 View
I have tried using BN-PAGE for mitochondrial complexes isolation. I have followed several protocols (Nature protocols-2006, JOVE-2017). But I experienced a problem with running the gel. The dye...
27 June 2020 5,587 6 View