Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
Isolating DNA from plasma using a column. DNA concentration on nanodrop is 5ng/ul. Unable to see any band in gel after 3-5 ul load no result in PCR amplification? what can be the reason?
18 November 2022 6,513 8 View
Hi, I would like to express a toxic construct in HeLa and 293T cells. It has to be easily inducible and absolutely not leaky. I did some research and only few promoters are available: Tet...
11 November 2022 6,639 0 View
Hi all, Recently I am working on protein purification. 1. affinity column(GST) 2. Add PSP enzyme to remove GST-tag(O/N) 3. GST-tag off 4. gel filtration However, the protein tends to form large...
08 November 2022 9,395 2 View
Hi, I'm working with human genomic DNA and try to analyse SNPs after PCR and digestion. If i get now 3 bands after PCR and restiction enzyme incubation for partly digested it could mean 2...
07 November 2022 6,244 5 View
Purified preps of IgG (via Protein G column) have yielded multiple bands under reducing conditions (mercaptoethanol, 5 mins, 95c) on SDS PAGE. Could you please help me identify the bands by...
05 November 2022 3,216 3 View
Just recently, I succeeded in making gold nanoparticles. Please assist with the preparation of gold nanoparticles for SEM and TEM.
31 October 2022 3,006 5 View
I am using 8% gel and Bio-Rad Precision Plus Protein Dual Color Standards. I have been loading 15ul of my ladder, and when I decreased the loaded amount to 5ul, I got the same weird appearance.
26 October 2022 7,622 3 View
Hello all! I have completed a test yeast expression for 5 different proteins, and I have taken a sample of the culture at 0, 24, 48 and 72 hours. 3 of my yeast cultures seemingly expressed a...
12 October 2022 3,308 5 View
We have two quant studio 3 real time pcr machines, no service contracts. One of the machines can run an experiment and the touch screen does work; however, none of the touch screen features work....
16 September 2022 6,557 0 View
Any lab uses a specific breeding scheme (Tg male or female to breed with nonTg parent) to generate Thy1-APPPS1 (Jucker) mice heterozygous pups to investigate amyloidosis? Please let me know!
18 June 2022 2,262 1 View
after pouring and drying when i am going to take out my sample then its harder whats the reason?
07 June 2022 7,392 3 View
Hello, I would like help in detecting and marking in western blotting. We in our laboratory do not have a chemiluminescence detection system. Would it be possible to detect chemiluminescence using...
03 June 2022 1,924 0 View
I ran a Coomassie and western blot using NuPage gels 4-12% and with my western blot had little to no signal. A few things: from the Coomassie, my gel was definitely overloaded, which I’ll fix in...
01 June 2022 6,853 0 View
The expression cassette includes a novel gene, two RNAi sequences and overexpression of a gene. I'm planning to place promoter and terminator sequence in between a novel gene, two RNAi sequences...
05 May 2022 6,546 2 View
I am using the following reaction condition Thermofisher trypsin dissolved in 50mM acetic acid (1mg/ml): 2 uL Protein sample: 50 ug (dissolved in 50mM ammonium bicarbonate, pH 8) Make the total...
29 April 2022 9,388 3 View
I'm planning to conduct APEGS assay for the first time. I got a protocol for the experiment, but have no idea how to prepare solutions used for APEGS assay. Materials using for the assay are not...
29 March 2022 2,370 4 View
acrylamide, urea, westernblot
21 February 2022 5,955 9 View
I performed the RNase footprint with an 106-nt RNA. However, my hydrolysis ladder showed poor resolution from the >35-nt bands (attached figure). I used the 8M Urea gel with 10% AcrylamideBis...
09 February 2022 6,130 0 View
I have been using an oxazine dye ATTO 655 for various purposes. Based on some mass spectra evidence, I wondered if the ATTO 655-maleimide reacts to the 0H-containing solvent like water or...
20 January 2022 6,613 3 View
Is there any way to determine the composition of biomass using thermogravimetry? I've tried DTG curve deconvolution using Origin software but was not able to get the correct composition. Can...
27 December 2021 1,756 4 View
My lab often uses these (https://www.coleparmer.com/i/whatman-6715-7502-polycap-tc-filter-capsule-pes-sterile-0-2um-1100-cm2-filling-bell/2970158) to sterilize large batches of medium. They are...
20 September 2021 7,278 0 View
Hello, can anyone please named few potential drug delivery technologies that you think are novel and unique. Our comapny is looking to buy few drug delivery technologies that can serve us to...
12 September 2021 1,351 14 View
I am working with platelets and getting blotches whenever I am running a gel. Why is it so? How can I resolve this problem? I am attaching my Coomassie gel image as a reference.
19 August 2021 7,100 2 View
I synthesized a bioactive glass by Sol-Gel process with composition SiO2-CaO-Na2O, in this process, I got gel after aging the clear solution. After condensation, for drying purpose, I placed a gel...
07 August 2021 7,980 4 View