Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
I'm looking for test methods, to test the stability of a suspension in a shower gel or an emulsion. All inputs are appreciated.
05 March 2020 2,035 5 View
I have Mini gel tank from life technologies, I want to use it for my serum electrophoresis. Does anyone have idea about how many percentage of ready to use agarose need to order for the SPE?
27 February 2020 8,959 3 View
Hi, I‘m having an issue with some ChIP experiments I have been doing with a GFP antibody and protein A dynabeads. I’m basically incubating overnight with my antibody and then using protein A...
20 February 2020 4,685 1 View
I am using a custom vector that has been provided to our lab. It has been sequenced and I have confirmed the restriction enzyme sites are present. I ran a digestion using HindIII and BamHI on...
19 February 2020 8,529 3 View
I ran western blot, for bax (20kd), 15%gel, 100v , then transfer overnight 40V 4 degree, I am attaching photos ( ponceu/ chemiluminiscence)kindly help me out , why bands get diffused-
05 February 2020 4,581 3 View
02 February 2020 3,137 2 View
I have got 4-5 extra bands in SDS gel after running a my eluted protein which was purified through Ni-NTA column. This means impurities are present. My question is, can I again run my protein...
02 February 2020 2,258 2 View
Dear, Whenever I run an SDS-PAGE, I get an unwanted vertical line appearing at the bottom of the gel. Does anyone have any idea why this happens? Any comments or suggestions would be highly...
02 February 2020 8,173 1 View
we are doing EMSA using thermo light shift kit and biotin labeled 150bp probe. We see a shift but also see a non-specific band in our control as well as samples. The probes is pcr amplified gel...
02 February 2020 2,205 3 View
Extracting DNA using CTAB method from 5 months old Cyperaceae samples for molecular identification. RNA bands are appearing on gel but DNA is always degraded. I tried 2-3 protocols but never get...
02 February 2020 909 1 View
I did a proteomic study of some rats liver extract, now i have some 2DE gel and i want to do a Diferentially Expressed Protein spot analysis. if there is a free software for 2DE gel analysis...
02 February 2020 1,083 3 View
02 February 2020 8,187 3 View
Hi, I ran 100ng of gDNA on a 0.7% agarose gel at 80V for 45min in 1XTAE buffer which was made fresh from a 50X stock which was produced 3 days ago. The samples were loaded in wells 3 and 5 where...
02 February 2020 292 3 View
I want to see a 16kDa band of my protein in SDS PAGE. Whenever i run the gel, the band in the upper part of resolving gel were very sharp and clear however the lower part where i supposed to get...
02 February 2020 6,397 2 View
Currently, i am thinking about GelAnalyzer software. Please help me with the analysis and band scoring. I have used 100 bp ladder. PIC 1 and 2 are different image.
02 February 2020 4,623 4 View
02 February 2020 8,332 3 View
I'm trying to purify my pcr product by using QIAEX II Gel extraction kit. According to the protocol, firstly i need to add buffer QX1 to the tube (contain gel slice that already been cut)...
02 February 2020 5,009 5 View
I did a temperature gradient PCR where I got specific band at 68 degrees.The extension temperature is also 68 degrees. When I run the product on gel I got a less intense amplicon for 68 degrees...
02 February 2020 8,558 1 View
Hai, I am using the concept of filling liquids/gels in the matrix of a photonic crystal structure. How can we calculate the effective refractive index of such structures? Thanks in advance....
30 January 2020 1,272 3 View
I m making 10 percent seperating gel but my both gel stacking and resolving are not solidify plz let me know how to resolve this issue
02 January 2020 8,298 3 View
If I have 3H-labeled samples on SDS-PAGE and stain them with silver stain, will they still be compatible with enhancing and fluorography methods?
01 January 2020 1,518 1 View
After FITC labelling proteins we should separate free FITC molecules from others. This stage is done with gel filtration. But now I lack access to gel filtration. I want to know if I can use...
01 January 2020 5,169 0 View
I want to extract aloe vera gel to use as a natural, harmless human extract to inhibit the growth of some fungi on food.
01 January 2020 5,588 1 View
Dear all, I am trying to find an alternative to southern hybridization for copy number detection in transgenic Arabidopsis. I have attempted the QD PCR protocol (T.Kihara ) for the protocol but I...
01 January 2020 4,637 3 View