Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
We know the capacity of the gel and the total amount of protein in the bacterial lysate from a Bradford assay. What we do not know is what fraction of the total protein is the his-tagged protein...
16 November 2020 4,922 2 View
Anyone has the experience in electroporation using Amaxa 4D nucleofector for Naural Killer cells from PBMC prep? I want to use the cell as source for iPSC generation. What is the efficiency? Thanks
10 November 2020 6,819 1 View
I'm studying a bacterial protein containing 2 cysteine residues. I subject the bacterial culture to either mock treatment, ROS treatment, and ROS+b-ME treatment, then harvest the culture to run an...
09 November 2020 4,438 1 View
I want to dissolve 3 mM HAuCl4 in 5 ml oleylamine. So, for this I have to make a gold chloride solution in DI water which is to be dissolved in oleylamine. But whenever I mixing oleylamine with...
06 November 2020 2,161 2 View
Dear all, I hope one or few of you would be able to give some feedback on immunoprecipitating membrane protein in yeast. I work with one integral membrane and one peripheral membrane proteins...
05 November 2020 9,561 4 View
Dear all, Hi I have strips of blue native gel, from chloroplast samples, which I have kept in fixation solution (10% acetic acid, 10% isopropanol, 80% water). Do you know if I run a second...
30 October 2020 5,619 1 View
Hello everyone, I am new in the field of PCR-RFLP and I am planning to use it for clustering yeast isolates. I am using HaeIII, HhaI and HinfIII to digest the ITS region and using GelAnalyzer...
30 October 2020 2,376 2 View
So I performed transformation on BL21 cells and then used those colonies to perform colony PCR. However results on the gel are just coming up as long smears. I did it twice , with the same...
26 October 2020 6,909 5 View
Hi all, I am having trouble with collagen type I as a scaffold for cells. I have a 3mg/mL collagen I solution with pH = 2. I am neutralizing with 1 M NaOH to pH ~7, then mixing with ice cold...
23 October 2020 8,672 3 View
I have found in a paper that the bulk modulus for Alginate gel is higher compared to micron size (approx. 50 um) beads measured with AFM for the same concentration. I am not sure what factor is...
16 October 2020 3,289 6 View
Hello everyone, I want to find out how the co-culture of two anaerobic bacteria influences each other in order to find competition and cross-feeding relations between them. My first approach is...
15 October 2020 403 1 View
The aim is to prepare a concentrated silica nanoparticle dispersion (si=70wt%) in a glycol medium with minimum water content. Method A: I dispersed silica nanoparticles (diameter 450nm) in...
15 October 2020 5,934 13 View
Hi ! I am starting my PhD studying the interractions between macrophages and the tumoral extracellular matrix and I am trying to design an experiment to assess the effect of the stiffness of the...
12 October 2020 990 1 View
I am doing an electrophoresis using a Tris-Acetate gel and the vendor offers a 1.0 mm and 1.5 mm thick gels. I just want some insight on the advantages. Thank you
08 October 2020 3,455 2 View
Would some one propose an effective method to measure the diffusion coefficient of a transparent solution in a transparent gel (agarose)? Thank you in advance.
06 October 2020 3,074 3 View
As I ordered DOTAP in the specification it was written that it is a powder. When we got do tap I realized that İt is a semi-solid state. I think that it has lost its stability. Am I right?
04 October 2020 5,792 2 View
Hi, everyone! I am really confused. From yesterday, my stack gel of BN-PAGE never polymerize. However, the same materials I used for making the running gels, which polymerize normally. I was...
03 October 2020 3,412 1 View
Both my marker and protein are stacking but they are not going through the stacking gel into the separating gel. Please help. My stacking gel concentration is 4% while the separating gel...
28 September 2020 8,280 2 View
how could we remove the sugar part from the lyophillized juice of fruits without disturbing the other hydrophilic secondary metabolites.
23 September 2020 8,902 2 View
Hi, everyone. I will want to know how to measure the thickness of bacterial cell wall after fixing with paraformaldehyde, did you perform this experiment? Any information provided, i would be very...
15 September 2020 6,715 2 View
Hello and good morning to all of you, hope you all will be fine, i have a question regarding gel electrophorosis, i was using SDS-PAG for phloem sap proteins, but i achieved the bands even lower...
14 September 2020 3,729 2 View
Hi everyone, In order to do CHIP experiment, I used some lysis buffer and micrococcus followed by sonication to digest chromatin of the cells. I did all the process of cross-lincking, quenching,...
12 September 2020 1,515 3 View
i am Iqra, i am doing research on purification of amylase i want to perform zymogram of amylase. anyone send me the protocol for native PAGE electrophoresis.
08 September 2020 4,969 3 View
I am looking forward to evaluating changes in the proteome level of mouse brain after drug treatment. I have done two trial experiments of MS-MS run with in-gel tryptic digestion and I also...
08 September 2020 297 3 View