Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
I'm trying my hand at Zn2+ Phos-tag gels. I have been working with 8% acrylamide, but my protein migrates through this very slowly. I attempted a 6% acrylamide and instead of waiting 5 hours for...
12 December 2019 6,649 6 View
Always when I run agarose-gels I get these droopy bands from my DNA ladder (and sometimes samples), and based on the sample bands I get in different experiments, the true sizes in the ladder seems...
12 December 2019 824 3 View
Looking at other questions and answers regarding typical cloning procedures (restriction-ligation), I notice almost everyone suggests to GEL purify DNA at several steps. For example ; after a PCR...
12 December 2019 7,732 7 View
I am following alkaline lysis protocol very carefully, with special attention to the correct pH of all the three solutions - TE with glucose, NaOH with sds and K-acetate with glacial acetic acid...
12 December 2019 4,855 7 View
How can I detect a specific protein within a protein mixture of blood serum by using western blot? I'm facing a problem to run the serum directly on the gel because it is very concentrated...
12 December 2019 7,749 9 View
This has really been quite frustrating. Every time I load my samples (20 to 45 ug per well), I see lines form in the resolving gel and they interrupt the run. The proteins just accumulate on that...
12 December 2019 7,464 10 View
I have used 2% DMSO in a PCR mix and after that I have purified the DNA using gel extraction kit. After purification I have subjected the product to restriction digestion followed by another round...
12 December 2019 1,893 2 View
I purified a bacterial genome and then done PCR with a universal primer. After that i ran the gel electrophoresis with a dna ladder, but i could not get the amplified gene of interest in my gel....
12 December 2019 6,461 5 View
We have isolated a bacterial endophyte that grows well in MS media and afetr some 20 days in culture without subculturing, we witnessed some clear, gel like,very viscous granules sticking on to...
12 December 2019 7,229 3 View
Hello guys, I have a serious problem with genotyping of IR flox mice. First I did according the Jackson Protocol: https://www.jax.org/Protocol?stockNumber=006955&protocolID=16534 It should...
12 December 2019 1,884 3 View
I subcloned the first two gibson fragments after a fresh gibson ligation, the products are joined from an 800 bp promoter and a 1,500 bp gene. I see that band. That band appears in the right...
12 December 2019 1,398 9 View
Dear all, I am now very frustrated about the cloning result. As I had undergone the TA clone and pick a white clone. I have also done the colony pcr (M13 reverse and T7 promoter primer) and a...
12 December 2019 9,490 7 View
11 December 2019 3,269 7 View
I'm making interactions between two proteins of similar molecular weights (30 kDa & 31 kDa). Is it possible to identify these two proteins (after interaction) on a gel?
11 December 2019 5,594 3 View
I am currently trying to troubleshoot why my gels are not showing anything at all except for my ladder marker bands. I have attached an example of a gel that I used to run previously with no...
03 December 2019 9,264 4 View
I downloaded the MRI breast database from cancer imaging archive but it does not include ground truth images
22 November 2019 3,252 2 View
Hi everyone, I am cross-linking collagen based hydrogels, and am looking for a method that I can use to evaluate whether the effects of the cross-linking on mechanical properties is due to actual...
21 November 2019 4,849 4 View
We are trying to express a protein following Invitrogen Pichia expression kit manual using extracellular vector pPIC9K in Pichia pastoris without any tag. The culture was grown at 30oC with pH 7...
11 November 2019 5,868 2 View
Hello , So i digested pBeloBAC11 using HindIII ( left the eppendorfs 1 hour in the water bath for the digestion process ). After that the sample was frozen at -20 C and next day a gel...
11 November 2019 1,108 3 View
I am working on generating 3D construct using L929 cells. I have encapsulated the cells in a hydrogel matrix. After 4 days the cells have increased in number, however, their morphology is...
11 November 2019 9,256 3 View
Hi, currently I am working on DNA extraction of bamboo leaves. This is the picture of the DNA extraction result.
11 November 2019 1,436 4 View
I have isolated mung bean's DNA using Qiagen DNA isolation kit from dried leaves of this plant. But I have obtained an additional low molecular weight band which I presumed to be of RNA but still...
11 November 2019 4,911 2 View
we isolated the DNA from various environmental samples and then did the PCR for 3 times. we also changed the primers that were fresh and of good quality but still got nothing. while the other...
11 November 2019 3,757 3 View
Hello I was performing the semi-dry transfer of my gel to the membrane for western blot What is the transfer buffer recipe that you guys use for semi-dry transfer? I saw a different recipe for...
11 November 2019 8,618 3 View