Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid... | Contact experts in Gels to get answers
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Questions related to Gels
Patient diagnosed with intractable major depression. Olanzapine treatment caused severe drug-induced Parkinsonian symptoms. Olanzapine discontinued 18 months ago and patient recovered from DIP....
16 July 2020 1,884 7 View
Recently, I'm having difficulties with my Western-blots and lectin-blots. Everything looks fine, until I reveal the membrane and it looks like it is divided in two halves, with the upper half...
16 July 2020 5,667 0 View
Hello, I'm recently facing with serious problems with my Western Blot experiments. I'm now doing this protocol since 4 years (same cells, same antibodies, same amount of protein loaded etc...) and...
10 July 2020 5,329 6 View
I have to score the gel images generated by ISSR markers. Could you please explain how scoring the gel images for ISSR markers?
09 July 2020 6,020 2 View
Want to set up a 2D protein gel system. Can anybody recommend a system to go for? We use a lot of Bio-Rad's protein gel systems, but don't know which one is best for 2D gels.
08 July 2020 6,458 3 View
I am studying the re-swelling kinetics of dried hydrogel films. I want to use a rheometer to follow the modulus decrease during the re-swelling. However, during the re-swelling both the thickness...
02 July 2020 1,132 5 View
I am looking for emulsifiers/surfactant commercially available to emulsifiy petroleum jelly. Having higher lipophilic value.
29 June 2020 4,492 0 View
I have tried using BN-PAGE for mitochondrial complexes isolation. I have followed several protocols (Nature protocols-2006, JOVE-2017). But I experienced a problem with running the gel. The dye...
27 June 2020 5,594 6 View
Hello, I have recently started reading about fermentation and inevitably stumbled across the description of the composition of the culture medium adapted to a given host organism. Here is a...
24 June 2020 7,975 0 View
I have prepared agarose gel, now i want to dissolve the gel completely in order to do the further analysis, I have put in the beaker and added some water to the gel and then I tried stirring at...
18 June 2020 3,965 10 View
It is observed that high molecular wt Or long chain length of oils result in increased droplet size of microemulsion in general (keeping other variables constant) . Can anyone suggest any...
09 June 2020 6,229 0 View
I wonder if anybody has an experience of sequencing PCR products where master mix contains green dye for gel loading. What if I will purify them with enzymes instead of gel extraction or...
19 May 2020 5,987 5 View
Hi, guys, I have a queation, please help me. I used air liquid interface method to do 3D culture. This is the first time to do it. the collagen gel prepared according to literature, 8 vol of...
14 May 2020 7,420 2 View
Dear researchers, I am recording my Western Blots on a Biorad Chemidoc System which results in one .scn file for the marker and on .scn file for the actual Western Blot signal. The coresponding...
06 May 2020 9,036 0 View
Hello everyone, In order to perform cell cycle analysis, I need to harvest cells from 6wp. I wonder what is the optimal volume of trypsin I should add to each well. I will appreciate any help in...
05 May 2020 6,188 0 View
I want to create MGDG (monogalactosyldiacylglycerol) reversed hexagonal phase, but I have a problem. I constructed a system that consists of two bilayers and an inner water layer between these two...
05 May 2020 7,805 0 View
I have a simulated trajectory in GROMACS of lipid, protein, and explicit water. I only have the .xtc output and the .tpr input for the production run. I want to calculate the energy of the lipid...
03 May 2020 8,769 0 View
hello, I have 3 questions- 1.In all publications i see that they used TBE buffer in EMSA (polyacrylamide gel), why? can I try in the binding reaction and running the gel with other buffers?...
02 May 2020 9,267 0 View
I am trying to prepare gel based hand sanitizer using WHO formulation with IPA. Water based hand rub is 80% alcohol, when I am replacing water by CMC gel solution of 2% it is not dissolving in...
30 April 2020 2,859 3 View
I am trying to minimise the energy for a DOPC lipid bilayer on gromacs 2020.01. I have done the following steps to prepare for energy minimsation: 1. Generated lipid bilayer using Charm-Gui...
30 April 2020 6,387 0 View
Please find attached a photo of the problem; After a simple Agarose gel electrophoresis of DNA with EtBr i find the gel "divided" in two parts. Analizing at the gel at 259nm it seems that the...
29 April 2020 2,500 7 View
Please mention the equations for PSD calculations from these methods. Also mention the software that can be used.
17 April 2020 3,768 0 View
Till now I've used SIFT, PROVEAN, POLYPHEN-2. What other servers are out their that analysis the deleterious effects of SNPs and their impact?
11 April 2020 1,810 5 View
In the place where I work we manufacture our own GelMa. However, lately we have been having some problems, so we need to characterize the degree of methacrylation of the Gel. Do you have any...
02 April 2020 371 2 View