What is the reason behind blotches in western blots?

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Have you seen the attached publication?

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Cloudy/high background western blot - can anyone help me?

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Rafia Mahmood

It may be due to improper transfer. Also, use a shaker for all incubation. Make sure there are no air bubbles trapped between gel and membrane.

Silvia Clapauch

Some authors describe it as an excessive amount of protein in the serum. Take a look at "Morin S et al. A GroEL homologue from endosymbiotic bacteria of the whitefly Bemisia tabaciis implicated in the circulative transmission of tomato yellow leaf curl virus. Virology. 1999;256(1):75-84."