Does anyone know a laboratory in asia where I can send mice fecal samples for microbiome profiling?

My study will require microbiota profile. Unfortunately, we dont have facilities and skills to do that or collaborators. So im thinking of sending the samples for analysis.

26 January 2018 7,462 3 View

Can you translate convert in vitro enzyme inhibition into in vivo dose in mice?

I will be doing an invitro study on lipase inhibition of an extract vs. orlistat using IC50. I will be using different concentrations of the extract to identify the concentration that is...

15 October 2017 4,399 3 View

Are elisa assay kits with 96 plates need to be used all at once? or can it be used in before and after studies with month-long intervals?

I will be doing a study in rats and I will purchase a 96 well assay for insulin. I will use only 30 rats and measure it twice.

09 October 2017 8,887 3 View

INS-1E cells don't adhere after thawing

I have a big problem with INS-1E cell line. I thawed 2 stocks of INS-1E cells (frozen in April 2010 in N2) and they simply do not adhere to the flask surface. Does anyone work with this cell line?...

25 June 2012 4,617 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

No title

01 March 2021 3,905 5 View