Dear Andres Alvarado Salazar, please have a look at the following documents. Aren't you looking for a denaturant gel? My Regards
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329804/#:~:text=Use%20ultrapure%20urea%20and%20mix,microwave%20and%20mix%20it%20gently.
https://molbio.mgh.harvard.edu/szostakweb/protocols/denaturepage/,kindly check this link.
https://www.protocols.io/view/preparing-and-running-a-tricine-urea-gel-7sxhnfn,u can follow this link.
http://cshprotocols.cshlp.org/content/2006/1/pdb.prot3793.full,this link may be useful.
Urea PAGE or denaturing urea polyacrylamide gel electrophoresis employs 6-8 M urea, which denatures secondary DNA or RNA structures and is used for their separation in a polyacrylamide gel matrix based on the molecular weight. ... A higher percentage of polyacrylamide resolves lower molecular weight fragments.Oct 29, 2009
https://www.ncbi.nlm.nih.gov › pmc
Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE) - NCBI
Dear Dr Andres Alvarado Salazar . See the following useful RG link: Article Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
Kindly see also the following useful link: https://www.protocols.io/view/preparation-of-denaturing-polyacrylamide-gels-and-nz3df8n
Also check please the following good link: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011971_Denaturing_PolyacrylamideUrea_Gels_TBE_Buf_UG.pdf
The following link is also very useful: https://deepblue.lib.umich.edu/bitstream/handle/2027.42/143785/cpnca03b.pdf?sequence=1
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