Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

No title

01 January 2021 5,831 1 View

What does it mean to set the measurement covariance matrix zero in Kalman Filter?

I am using a linear Kalman filter for estimating the position using multiple IMUs. My model is adding the accelerometer readings' double integration onto the previous positions. The state vector...

09 July 2020 5,730 2 View

Lentiviral transfer plasmid - constitutive RFP with puromycin selection marker?

I was able to find 3 plasmids at Addgene (below) that came close to these specifications but they're not quite there. Can anyone point me to a LV transfer plasmid expressing constitutive RFP and...

26 November 2019 240 2 View

I have cholesterol and derivatives and I should dissolve them in solvents. In which solvent can I dissolve the desmosterol?

I bought the desmosterol from Sigma-Aldrich and there is no explanation about it. The amount of desmosterol that I have is 5mg.

14 February 2018 1,239 3 View

T4 Bacteriophage Genomic DNA

Hello everyone. I am trying to obtain T4 genomic DNA however, I couldn't find any source to get one. I am not allowed to handle the phages in my institute therefore I would just need the genomic...

29 May 2017 1,060 1 View

How can I co-culture two adherent cells ?

Hi, I want to set up an experiment to analyze ability of THP-1 derived macrophages to kill MC7-7 cells. But both of them are adherent cells, which makes it difficult to culture them in one plate....

11 October 2014 5,671 8 View

Has anyone coated flasks for cell adhesion?

Has anyone successfully cultured cells with flask coating (BSA, collagen and fibronectin)? How can we coat flask with this mixture, what is the coating incubation time? What is the storage...

24 June 2014 6,910 7 View

Do you have any suggestions about freezing primary fibroblast cell lines?

Primary fibroblasts

18 January 2014 7,800 13 View

Is CoCl2 harmful for hypoxia induced exosome content?

When we study with hypoxia induced exosomes, could CoCl2 be harmful for exosome content in the cell culture media?

01 January 1970 7,586 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View