I am looking for polymers/biopolymers with high hydrophobicity and high gas permeability. Can any one recommend any types of polymers/biopolymers?

I am looking for polymers/biopolymers with high hydrophobicity and high gas permeability. Can any one recommend any types of polymers/biopolymers?

25 November 2020 5,887 3 View

How to use Incremental/Online learning in face recognition?

I am trying to implement incremental/online learning for a facial recognition application. I've trained a model on a dataset and it works perfectly fine, however, I need to capture new...

23 September 2020 3,819 5 View

How to Fill Up volume in CST MWS?

How to Fill Up volume in CST MWS Hello everybody, Does Anyone know how to fill up a generic volume with a certain material using CST Microwave Studio Software? How to connect a curved plate to...

07 February 2020 4,427 1 View

How to use a CNN model saved as .mat file in python?

Hello everyone, I am going to use a FER2013 fine-tuned pretrained VGG-Face model to extract some features from facial images. Actually, I have the weights and layers of this model saved as a mat...

21 January 2019 4,387 2 View

Which CD20 expressing cell line is better?

Hi there , we are working on antibodies against CD20 antigen ( tositumomab, ofatumumab, Obinutuzumab ) and need a cell line which overexpressing CD20 . as far as I am concerned, Daudi , Ramos...

07 November 2018 1,015 3 View

Can I use SLA1 instead of XHO1?

Hi there. I want to know is it possible to use SLA1 Enz instead of XHO1? My gene of interest is CD20 And I'm working on PSpax, PMD2G plasmid and PCDH. Any suggestion or comment would be...

31 August 2018 7,838 3 View

PCR troubleshooting.what should I do to solve the problem ?

29 August 2018 1,651 6 View

Any suggestion or comment in regard of PCR troubleshooting ?

20 August 2018 150 10 View

Hi.How much blood is needed to gain enough lymphocytes to do RNA extraction?

How many PBMC can be isolated from 10 ml of blood from a healthy donor?

19 July 2018 7,153 3 View

Why does my Blue native gel doesn't run completely?

I have been running blue native gels for my chloroplast samples, the problem is that the gel won't run completely. I have seen gel pictures in different papers that the whole sample runs really...

15 January 2018 4,291 2 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View