We are using MS5A column for H2 and O2 detection during photocatalysis experiment. Is this column afraid of water and we should not use manual injection and only use autosampler.
but many researchers using manual injection and take samples from the reactor filled with water?
What is correct as we don't have autosampler?
Jaideep Malik: Please contact someone knowledgeable and local regarding proper operation of the instrument. You may damage the instrument if used without having someone with training supervise or assist you. * Molecular-sieve coated (or based) columns are highly susceptible to contamination by water (and CO2). They should not be used for water or CO (CO2) analysis. Column deterioration and damage (drifting retention times are often a first sign of trouble) often result from the introduction of water into the gas stream. SAMPLES for analysis MUST BE DRY to use with molesive type columns. If contaminated with water, you can try to dry the column out per the column manufacturer's recommendations (e.g.. 310C for ~8-hours, but as their are many types of molesive columns, please refer to specific manufacturers recommendations).
Notes:
Unless the injection method or technique used are performed incorrectly, the device used for injection (manual vs automated) should not cause an issue, while use of the wrong mode of injection may result in problems. *Use split mode to prevent the water from being directly injected onto the column (Insure heat vaporization is set properly and only inject a small portion using a high split ratio).
Before using any column, please read the column instruction material provided with it for proper use and limitations (a lot of valuable material can be found in the manufacturer supplied application notes and instructions for each column).
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