Can I use carbo-free blocking solution in place of normal goat serum?

I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...

07 February 2021 7,520 6 View

Can problems with blocking cause high background and weak antibody signal during immunofluorescence staining?

I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...

10 November 2020 3,620 7 View

Does anyone work with femoral and tibia radiography on rats or mices?

I would like to know what protocol followed for animals undergoing some treatment that induces some type of osteopenia / loss of bone density.

13 July 2019 1,731 2 View

What are the best research-based tools to teach pronunciation of L2 segments to second language learners?

Last year I attended an International Conference at the University of Utah about the teaching of pronunciation in second language learning and teaching. I notice that there was a general...

23 May 2018 5,599 2 View

How is your research likely to improve the approach to measuring performance quality?

I would be very interested in reading your publications related to measuring health and safety/ quality/ operations management performance - especially those related to recommendations for...

17 February 2017 7,501 2 View

Statistical Data: Experimental Design (teaching, how many students, control and normal group, definite conclusions)?

An innovative proposal, so say a new way of presenting contents or a small book with innovative ways of creating reasoning patterns: What do I need to have to prove to others, in a fully...

30 May 2016 9,386 3 View

Can you suggest a good assay to determine paracellular permeability in epithelial cells?

I am establishing a bovine oviductal epithelial cells culture using inserts of 0.4um pores and I am trying to get the cells polarized with this system. I would like to use the permeability assay...

16 January 2014 7,820 18 View

Does anyone know how test strips measure current for glucose detection?

Do they work by lateral flow and if not is it possible to make a lateral flow device with something like a test strip?

23 October 2013 7,767 4 View

How would you teach occupational safety and health management to social science classes compared to/contrasted with engineering classes?

I am planning to teach OSH to management students for the first time. I have had much experience teaching this field to technical students (engineering students, operators, etc.) and I am thinking...

01 January 1970 9,065 6 View

When doing research in a small island context, how can you show relevance to a more international/ developed nation audience?

Should papers be written from the context of a developing nation, rather than just an island? How can I show the relevance of my research to the USA, UK, the EU? Do I use my country's demographic...

01 January 1970 7,564 6 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View