One way ANOVA followed by Dunnett's post hoc test?

I am doing a research in biotechnology where I had my animal studies results. The SD values of the body weight of the animals were conducted. I need to find out the statistical significant values...

23 September 2020 8,722 8 View

One way ANOVA followed by Dunnett's post hoc test?

23 September 2020 5,978 3 View

Reduction in islets size due to Focal cytoplasmic vacuolation?

When the rats were induced diabetes with the administration of Streptozotocin Section from pancreatic islets and acini shows cytoplasmic vacuolation. Islets are small in size with reduced in the...

09 September 2020 9,282 2 View

How to Integrate of Sumo and Veins?

Hi everyone, I have installed omnet 4.3, sumo 0.21.0 and veins 3.0 (This version was necessary as I want to work with NETA framework) . I was able to run the example from Veins and also I...

07 January 2020 2,824 1 View

Psychometric tests and student profiling/competency mapping

Hi all, I am designing a student profiling system (in university level technical education) in which student competencies are mapped and used for further intervention. I want to test achievement...

31 May 2017 1,552 11 View

How to do microbial extraction for isolation and identification of secondary metabolites?

20 April 2017 6,010 4 View

Will overloading an inductor in buck converter affect its performance?

When we increase the load current in buck converter, capacitance and inductance values needs to be over dimensioned. What happens to its characteristics if the same value of inductor is retained?...

03 November 2016 440 7 View

Do you always do a no RT control for every RNA sample that has been independently extracted and from which cDNA has been prepared cDNA?

We are just starting to do real time RT-PCR and I have question about controls for real time RT-PCR. Do you always do a no RT control for every RNA sample that has been independently extracted and...

20 September 2016 2,535 2 View

What type of excel (2007) datasheet could I join to Arcmap (version 9)?

I have an empty country map of settlements and I would like to join a database of settlements datas. I used dbf format, but since I have new 2007 office I can't join files.

27 February 2014 2,078 1 View

Why is CTAB is more preferred in isolating DNA from plant sources?

I need a comparison with SDS.

25 December 2013 1,334 12 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View