I've designed media for CHO cells, without Glutamine! But when they're in large scale, the cells were damaged and decreased their viability. What's your refer ?
Hello Negar Divandari
You may use Glutamax instead. Glutamax, which is L-alanyl-L-glutamine dipeptide could substitute for L-glutamine in your cell culture media, resulting in efficient energy metabolism and high-growth yield, without the detrimental effects of excess ammonia on your cells. Moreover, Glutamax does not degrade into ammonia during prolonged incubation. The mechanism of dipeptide utilization involves a gradual release of peptidase during culturing, which allows a gradual hydrolysis of the dipeptide in the medium.
I had used Glutamax for CHO cells to produce therapeutic proteins and grown them at high cell densities in serum free suspension culture. The CHO cells had shown improved growth, protein expression and decreased ammonia accumulation.
L-glutamine is preferably not used in cell culture media as it spontaneously degrades, generating toxic ammonia and pyrrolidine carboxylic acid as by-products.
Best.
Hello Malcolm Nobre Thanks for your answer, but when I using etch item that Glutamine derivatives, I will have problem...However, thank you again
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