The study of the properties and action of enzymes. | Contact experts in Enzymology to get answers
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Questions related to Enzymology
I'm going to start to work with this system and I have a question, can I prepare the Ascorbate and store it in the fridge for a while or its better to prepare all the time fresh for the assay? Im...
05 May 2013 6,204 2 View
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05 May 2013 7,795 6 View
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05 May 2013 4,693 9 View
Can anyone tell me the name of any bacterial enzyme that can be assayed feasibly with a suitable substrate in the lab? Protocol for that assay?
05 May 2013 2,309 5 View
Restrictive endonucleases and ligases are critical tool enzymes for DNA manipulation and genetic engineering. If you have similar tool enzymes for protein manipulation and engineering, it will be...
05 May 2013 6,843 1 View
I tried looking on the net and I couldn't find the corrct answer. Why do we use wavelength of 540nm in the spectrophotometer?
04 April 2013 3,025 1 View
In Alpha glucosidase assay does PNPG (from SRL) degrade readily if kept at room temperature for about 2-3h?
04 April 2013 720 1 View
Pyruvate dehydrogenase complex.
04 April 2013 4,386 0 View
Can alpha glucosidase and alpha amylase work at room temperature and at 37*C? If so why? The specifications for these enzymes are that they work at 37*C. But number of papers have modified their...
04 April 2013 2,600 3 View
In TBARS experiments
04 April 2013 4,510 1 View
I am working on the literature for the maltodextrin phosphorylase. I found that the E.Coli K-12 Mg 1655 strain is a more potent strain. I want to confirm the potential of the strain through PDB,...
03 March 2013 479 0 View
I determined the binding constant of an amino acid substrate for an enzyme to be 5.0 mM, what does this number mean in terms of the affinity of the enzyme for the substrate?
03 March 2013 6,517 2 View
I have a lot of difficulties measuring succinate-CoA ligase (with ATP/ADP or GTP/GDP) in fibroblast homogenate or in mitochondria isolated from fibroblasts. I tested all the published methods (at...
03 March 2013 914 7 View
Is there a protocol for MDA quantification (as a lipid peroxidation product) using the same buffered plant extracts used for anti-oxidant enzyme assays, instead of using homogenates of plant...
03 March 2013 1,519 17 View
To enhance the yield of the extraction of aromatic plants, some enzymes are used as a pre-treatment of the vegetables to transform aromatic compounds like monoterpenyl glucosides into free volatil...
03 March 2013 9,329 0 View
This is a follow up question to the question asked by Sriharsha Prabhakara on WHY THE BODY PREFERS GLUCOSE AS ENERGY SOURCE. Of course, I know about the stereoselectivity/specificity of receptors....
03 March 2013 5,723 29 View
I want to isolate bacterial culture for enzyme production. I want to retard the growth of the fungus in media . Which is the best anti-fungal antibiotic which can be used in the media?
02 February 2013 224 15 View
Google
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02 February 2013 9,897 2 View
Are there any human enzymes which can act similar to the bacterial haloalkane dehalogenases? For example can reactions leading to the same products (haloalkane metabolised to hydroxyalkan) be...
02 February 2013 1,649 5 View
Co2+/ Mn2+, Metal content determination.
02 February 2013 6,622 0 View
I need to homogenize my rat's liver to obtain crude enzyme. Usually I homogenize it with phosphate buffer and salts like KCl and MgCl2. It has worked, but I am afraid that the salts could be...
02 February 2013 5,444 23 View
Determination of the activity of some Exoenzymes produced by Vibrio cholerae such as Protease, Elastase, Lipase, Lecithinase and Hemolysin?
02 February 2013 6,479 0 View
Bound clarified lysate for 1hr @ 4C in 150mM Tris, 50mM NaCl, 40mM Imidazole. Then spun down, and when I added the same buffer to wash, the protein crashed out (lots of white gunk & precipitate)....
02 February 2013 253 17 View