The study of the properties and action of enzymes. | Contact experts in Enzymology to get answers
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Questions related to Enzymology
If I know that the free energy barrier of the first step of a enzymatic reaction has increased (And I know by how much) but the other steps remain unchanged may I infer from this any kind of...
23 January 2016 8,693 6 View
Dear Researchers, I performed Tyrosinase purification from bacteria broth medium , but I have difficulty to purify this enzymes because of the presence of melanin. Does someone have any idea on...
21 January 2016 3,433 2 View
I know that protease inhibitors are typically dissolved in water, alcohols or DMSO, so the suppliers normally don't test for acetone solubility. Anyway I'm looking for protease inhibitors (serin...
01 January 2016 6,756 3 View
while plotting velocity vs substrate graph...initially the velocity increases and then it starts saturating but not completely saturated..due to the solubility limit of substrate I can't increase...
20 December 2015 878 12 View
12 December 2015 434 13 View
A genomic study has shown that an enzyme was about 40 times upregulated in the liver. How to estimate the maximum amount of substrate, knowing the its molecular mass?
11 November 2015 392 11 View
Hello everyone! I am a newbie in enzyme kinetic parameter. I'd like to determine enzyme kinetic So I set my experiment as follow: using D-galactose as substrate at 8 different concentrations ( 5,...
11 November 2015 6,281 10 View
I could not find the necessary information anywhere. Thanks!
11 November 2015 8,912 2 View
10 November 2015 8,035 2 View
PDB provides ligand information, but what about the proteins not submitted to PDB, is there any way to get active site information with out using ligand ??????? Please suggests online tools or...
10 October 2015 8,792 5 View
08 October 2015 5,739 4 View
Where can I buy (or order of synthesis) these inhibitors of sialidases: N-Acetyl-3-fluoro-neuraminic acids or 2,3-difluoro-N-acteylneuraminic acid?
09 September 2015 6,986 2 View
Currently I am working with alpha amylase assay using DNS method but I have a problem with the colour which is not changing even I used high concentration from the phenolic standards (Trolox and...
19 August 2015 7,171 12 View
T50 means the temperature that an enzyme lose 50% activity. Tm is the melting temperature of an enzyme. But the curve about T50 and Tm is highly similar. What's the specific differences between...
08 August 2015 1,010 4 View
08 August 2015 3,828 13 View
Am trying to work on this topic 'effect of pH, temperature and metal salts on the stability and activity of alpha amylase produced from Rhizomucor pusllus'. Just need a carbon source for the...
08 August 2015 8,168 3 View
03 August 2015 5,605 3 View
I have a doubt in calculating the activity of immobilized cellulase i've used the formula Activity of cellulase (μmol/ml min) = 1000 w/Mvt; where, w is the amount of glucose produced, M is the...
13 July 2015 8,022 5 View
07 July 2015 207 6 View
I'm helping in a project that aims to produce and immobilize the laccase enzyme from P. ostreatus, but we have not got a good immobilization protocol yet ( adequate concentration of glutaraldehyde...
14 June 2015 5,011 10 View
.while i think that DTT affect the rate of enzyme reaction. so maybe DTT can not solve our problem
28 May 2015 8,030 4 View
How to get primary plots of Relative Activity (%) vs Time point at respective temp? Secondary plot of ln V vs 1/K. During assay, what has to be changed, whether the time point for enzyme...
22 May 2015 6,766 4 View
Both enzymes belong to the trypsin family of serine proteases and both have a substrate specificity for argynyl and lysyl residue-containing substrates. I know that PAR 2 has a SKGR34↓S35SLIGKV...
21 May 2015 6,989 3 View
I tried docking an enzyme which is randomly mutated in order to improve the catalytic efficiency. Then tried to find best catalytic efficiency among all the mutated versions of the same enzyme...
05 May 2015 7,158 14 View