The study of the properties and action of enzymes. | Contact experts in Enzymology to get answers
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Questions related to Enzymology
I am looking for an enzyme that can hydrolyse a fucoidan polysaccharide into oligosaccharide units.
08 August 2014 4,964 1 View
I am trying to precipitate a bacterial cellulase produced on a lignocellulosic hydrolysate as the first step in the purification of the enzyme. I did step-wise ammonium sulphate fractionation at...
08 August 2014 4,506 18 View
Lignocellulose is a renewable biomass which is widely available in nature. It contains cellulose (glucose), hemicellulose (xylose, arabinose, mannose etc.) and lignin. How one can analyse a...
08 August 2014 5,334 2 View
How does one quantify total cellulose, hemicellulose, and lignin in biomass before pre-treatment of lignocellulosic biomass?
08 August 2014 9,163 23 View
How can enzymes be selectively purified with the help of this technique?
05 August 2014 2,944 2 View
I need PH optimum for enzyme for doing a good dialysis, so I determined PH and temperature optimum by impure enzyme. Is there significant difference with pure enzyme?
07 July 2014 6,843 7 View
in the estimation of Catalase the method assay K value, what is the equation to convert it ti IU.
07 July 2014 2,572 0 View
I have a a tripeptide which was isolated using prep LC. I have determined its accurate mass and empirical formula using LC-QTOf-MS. I went further and did acid digestion which has enabled...
07 July 2014 5,680 3 View
I was wondering if there are organisms lacking catalase. I know that Plasmodia and Schistosoma species do not possess this enzyme. Thanks.
27 June 2014 548 8 View
06 June 2014 3,385 7 View
Plant material extract was prepared in PCA. Then, it is treated with Potassium thiocynate and ferrous ammonium sulphate. OD at 480 nm. How to calculate the amount of H2O2 in the plant extract?...
06 June 2014 7,895 3 View
My recombinant protein, cellulase, produced in E.coli. I remove small domain from it. I think when my protein produce in high concentration, its activity is reduced.
06 June 2014 9,953 3 View
I am working on enzyme production and want to study its kinetics. To stop an enzymatic reaction I need to add KCN. Due to potential toxicity, I don't want to use KCN, so please suggest some other...
06 June 2014 2,440 11 View
I'm designing an assay to test for release of pyrophosphate due to dihydropteroate synthase-like enzyme activity using malachite green reagents. One of my substrates is enzymatically catalyzed and...
05 May 2014 5,181 11 View
I am trying to solve an Enzyme structure. After series of refinement the R factor is 25 and R free is 31. The structure is almost complete. The Ramachandran plot looks good with just 1.5% outliers...
05 May 2014 9,719 3 View
I attached the zymogram image. The top line is MMP-9 giving distinct band. But the MMP2 band is like a straight line across the gel both latent and active forms even in the marker lane. Could any...
07 April 2014 9,349 5 View
Many enzymes have preference for L versus D or R versus entiomers. Is there a precendent in the literature for changing, engineering by amino acid alteration, a D-selective into an L-selective enzyme?
03 March 2014 1,671 3 View
Aebi H. Catalase in Methods of Enzymatic Analysis ed.1984
02 February 2014 8,157 2 View
I have a protein that contains an active site residue at the end of an alpha-helix. It has long been postulated that the dipole moment of this helix contributes to its activity. I want to compare...
02 February 2014 345 2 View
I need to know if I can estimate the amylase enzymatic kinetics. I have data of absorbance and concentration and I have a standard curve of glucose (DNS) and starch (iodine solution).
02 February 2014 6,850 2 View
For a DMPD free radical chelating assay.
02 February 2014 7,343 1 View
I am working on a large GTPase (purified from E. coli based on His tag) of which I am trying to determine its kinetic properties. I have tested its activity at different GTP concentrations (100,...
01 January 2014 974 20 View
I am looking for promoters that are strong in Bacillus as expression hosts. I found only xylose-inducible promoter and P43 (a strong constitutive promoter, Wang et al., 2004 Biotechnology &...
01 January 2014 6,292 3 View
To study enzyme activity and purification of same.
12 December 2013 5,593 5 View