The study of the properties and action of enzymes. | Contact experts in Enzymology to get answers
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Questions related to Enzymology
I am using INFT in vitro experiments. But i am worried about the basal dilution have some effect on my gene of interest. Now i am to use deactivate or inert INFT in my dilution solution. I can not...
05 May 2015 6,824 0 View
How to get primary plots of Relative Activity (%) vs Time point at respective temp? Secondary plot of ln V vs 1/K. During assay, what has to be changed, whether the time point for enzyme...
05 May 2015 1,546 4 View
Both enzymes belong to the trypsin family of serine proteases and both have a substrate specificity for argynyl and lysyl residue-containing substrates. I know that PAR 2 has a SKGR34↓S35SLIGKV...
05 May 2015 8,959 3 View
.while i think that DTT affect the rate of enzyme reaction. so maybe DTT can not solve our problem
05 May 2015 269 5 View
In general lipase protocol- Lipase activity assayDetermination of liberated free fatty acid was measured by colorimetry method (Kwon and Rhee, 1986) using olive oil as substrate. The reaction...
09 April 2015 8,824 3 View
I'm working with a beta-glycosidase from a hyperthermophilic archaeon. Eventhough it is highly thermostable, its kcat values are very low. So we tried to mutate some catalytic site and non...
04 April 2015 242 7 View
I would like to know the cleavage site of the pre signal sequence of the Kallikrein-related peptidase 7. Thank you in advance.
04 April 2015 5,468 1 View
04 April 2015 3,660 3 View
I am measuring Ki for the inhibitor I have synthesized. I have finished all the experiments and need to plot the results to know if its a competitive inhibitor. I have never used Graphpad Prism....
30 March 2015 8,097 3 View
I am using Bacillus subtilis as a source for cellulase production grown on 0.1% CMC (Carboxy-Methyl-Cellulose) media. And now I want to detect the presence of cellulase using 0.1% congo red stain...
27 March 2015 9,536 6 View
In case of using crude enzyme samples, which we knew the properties of the crude enzyme is not pure and heterogenous. Is it better to use the DNS (with rochelle salt before) or use the DNS reagent...
09 March 2015 9,853 4 View
03 March 2015 7,975 7 View
Hi! I am measuring the activity of my enzyme of interest through the decrease on the absorbance at 340 nm (to detect the disappearance of NADH). I am measuring every minute for 8 minutes, and I am...
03 March 2015 2,909 5 View
03 March 2015 7,058 7 View
They seem to simply be plotting the rate of formation of product at multiple substrate concentrations, however they are well below the enzyme Km (they only go to 60uM when the Km is approx. 1mM)....
03 March 2015 5,247 3 View
I need advice to perform hydroxyproline extraction from bovine and porcine hides, in order to estimate collagen content.Which is the most efficient and reliable method? I found some literature on...
03 March 2015 9,039 8 View
03 March 2015 1,316 5 View
I am working with a hydrolytic enzyme. Is it possible that it can act properly without a buffer?
03 March 2015 760 9 View
I am going to use T-PER tissue extraction (Thermo-Fisher) buffer with Roche ultra Protease and Phostop to extract phospho-protein from mouse spleen and liver. I will collect fresh tissue in buffer...
03 March 2015 8,800 4 View
The cheapest way to immobilize enzyme having following property 1. should be in powder form 2. The powder should be soluble at pH 6-7 3. should be eatable. 4. activity loss should be minimum.
28 February 2015 9,802 7 View
How to find the active site(s) in a drug target enzyme for molecular docking? I want to predict possible or confirmed active sites in the following enzymes Adenylosuccinate lyase PDB (4MX2) N-...
25 February 2015 8,990 4 View
I am trying to purify my enzyme from plant source. I used 0-80% ammonium sulphate, can I use gel filteration using sephadex G-50 resin to desalt my sample instead of dialysis. As dialysis leads to...
09 February 2015 1,762 4 View
02 February 2015 9,345 8 View
My initial search shows a very wide variation in normal values. Thanks
02 February 2015 8,274 6 View