The study of the properties and action of enzymes. | Contact experts in Enzymology to get answers
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Questions related to Enzymology
My enzyme is a monomer but sometimes exists as homo-oligomer. The kinetic plot of the enzyme was hyperbolic. The current project on the enzyme involves incubating the enzyme with some compounds at...
12 January 2017 8,919 7 View
I wanna know with there is any osmolyte or other way to more activate a enzyme....
20 December 2016 8,467 6 View
Dear everybody, I'm looking forward your answers of this issue. Thanks in avance.
11 November 2016 1,064 4 View
I wanted to see if people had experience and success with this kit without the use of a liquid dispenser. Currently, I have had success with doing the direct enzyme + ATP and observing a good...
08 November 2016 3,333 4 View
I would like to make catalase activity standard curve, but i still fail. I would like to know protocol of making catalase activity standard curve.
07 November 2016 7,344 2 View
I'm doing a research on bromelain enzymatic activity in simulated gastric fluid (and also presence of other proteases). I've read up on previous In Vitro Study of Bromelain Activity in Artificial...
10 October 2016 4,463 2 View
i have to calculate activity of immobilized (using sodium alginate) lipase using pNPP assay
24 September 2016 9,823 11 View
09 September 2016 7,579 12 View
for example...How to deal with the source of the enzymeMethod of purificationThe type of materials that used in the purificationThe degree of purification...etc..
09 September 2016 836 6 View
Please who can help me with best production medium for Laccase apart from the one proposed by Tien and Kirk, 1988. Thanks
09 September 2016 3,051 2 View
After receiving comments from reviewers on a manuscript on the above mentioned subject, the reviewers claimed that aerobic denitrification has been reported to produce nitrous oxide, of which I...
25 August 2016 6,396 4 View
I need a pharmacological protocol to induce acetylcholinesterase over-expression in mice brains, in order to use the brain homogenate as enzyme solution.
21 August 2016 6,342 2 View
I am planning to study enzyme kinetic on acid phosphatase activity on isolated phosphate solubilizing bacteria which have been isolated from paddy field soil with pH ranging between 5-6.5. Since i...
11 August 2016 8,552 3 View
If i want to perform any enzyme activity how to select the substrate concentration in 3 mL reaction mixture? and if there is any donor is present in 3 mL mixture how to select the concentration of...
08 August 2016 698 3 View
I am trying to measure the amount of cholesterol in cell membranes but I have seen that the Amplex Red Cholesterol Assay kit is not suggested for use on cell lysates. I also don't see any specific...
08 August 2016 7,076 2 View
We are conduction some experiments to test Glutathione-S-transferase (GST) activity towards 1-chloro-2,4-dinitrobenzene (CDNB). We used three control wells where the enzyme solution was replaced...
08 August 2016 5,457 1 View
08 August 2016 3,042 5 View
08 August 2016 7,109 3 View
08 August 2016 10,054 2 View
08 August 2016 8,348 1 View
I am trying to produce Taq. This should be fairly straight forward, but something is going wrong, and I would appreciate some help troubleshooting what could be going wrong. I have the pAKTaq...
05 August 2016 6,135 8 View
How long can a serum be stored at room temperature (15-25 C) so that the enzyme activity is intact? The enzymes of concern are AST, ALT, ALP, LDH, amylase etc..
02 August 2016 4,308 2 View
Hello Everyone! I am going to look PPO and POX enzyme activity in fruits. I am getting confuse to make a standard curve. Which reference substances should be taken while making a calibration curve...
20 July 2016 2,596 12 View
I am now carrying out anti diabetic assay in which performing enzyme inhibition assay. further i want to detect which type of enzyme inhibition assay is that. So can anyone suggest some protocol...
07 July 2016 7,027 3 View