Can anyone tell me the name of any bacterial enzyme that can be assayed feasibly with a suitable substrate in the lab? Protocol for that assay?
Dehydrogenases are the easiest. All you need is NAD or NADH and the substrate and you can assay it in a spectrophotometer. If you want I can give you the protocol.
@ Siddhartha Roy:- Thank you so much sir for your suggestion. I shall be highly obliged if you can give me the protocol for that assay. I have one more question. Is there any assay for measuring the helicase activity?
For helicase assay, please go to google scholar and type "helicase assay". The first three articles will give you three different assays. Each of them relies on different principles and the choice will depend on availability of chemicals. For any dehydrogenase assay, again go to google scholar and type "dehydrogenase assay methods enzymol". You will get citations of really old (but gold) methods in enzymology articles on methods of dehydrogenase assay by pillars of Biochemistry (Ochoa et al.). They are simple yet still valid today.
You can extract most common enzymes like; alpha amylase and alkaline/neutral protease from Bacillus subtilis and protocols are very easily availbale on net. For amylase you can use DNS method using soluble potato starch as substrate and for protease you can use casein as substrate.
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