I want to isolate bacterial culture for enzyme production. I want to retard the growth of the fungus in media . Which is the best anti-fungal antibiotic which can be used in the media?
I've used Fungizone with good results.
http://products.invitrogen.com/ivgn/product/15290018
We use Amphotericin B to avoid fungal growth in mammalian cell culture but not sure of bacterial culture.
Amphotericin B is usually used for cell cultures but to isolate bacteria i suggest cycloheximide. Its commonly used in microbial media to suppress fungal growth.
http://www.sigmaaldrich.com/catalog/product/fluka/18079?lang=en®ion=AU
Amphotericin B as well as Cyclohexamide both I have used and they worked well for me. But sometime we used more quantity (>50 ng/100 mL) to avoid fungal contamination.
Cycloheximide as well as benomyl are working well individually to retard Eukaryotic growth including fungi.
I recommend use of Bavistin for general purpose.....For isolation, use of Cyclohexamide works satisfactory. Concentration can be adjusted depending on source and type of sample.
For the record: Fungizone = Amphotericin B.
If you want a good wide-spectrum anti-contamination additive try:
https://products.invitrogen.com/ivgn/product/15240062?ICID=search-15240062
I also recommend Fungizone, it works with embryo/cell culture media. You may find useful this tool to select the right antibiotic. http://www.labtools.us/antibiotic-selector/
amphotericin B (2.5 g/L) this concnetration we use in our lab.
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