How can I convert microM / gram of tissue for TMP mg / kg sample?

Luiz Claudio Miletti @Luiz_Claudio_Miletti

04 April 2013 1 5K Report

In TBARS experiments

Please clarify the full form of the abbreviations "TMP" and "TBARS".I have experience with TBARS, but I do not know whether your TBARS and my TBARS are same or different. Once clarified, the remaining part is expected to be easy.

Badges
Science topic

More Luiz Claudio Miletti's questions See All

Could someone explain to me why?

I'm using Triton X-114 to purify proteins membrane I'm trying to purified GPI anchors proteins.The analysis by mass spectrometry / bioinformatics reveals several cytosolic proteins.

04 May 2016 8,653 5 View

What type of inhibition does a Ca2+ chelator have?

As I remember according the classical enzymology there are four types of inhibition: Competitive, Non-competitive, Uncompetitive and allosteric Inhibition . There is an enzyme with Ca2+ as a...

21 March 2024 7,334 4 View

Studying history of enzymology and enzyme discovery?

i am new to enzymology and want to study the discipline through history based books (i,e, how was the Kreb's cycle discovered, methods used then, etc). what are your suggestions to achieve...

07 April 2023 544 2 View

How to calculate enzyme's energy of activation without rate constant (+excel)?

I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...

25 December 2020 9,030 3 View

What is apparent Km Value of an Enzyme, Is it different from usual Km value and Why it is important?

It is very much confusing when some papers use this term "apparent Km of an enzyme even if they use single substrate ? Not at all clear ! Please help me understanding this. Thanks in advance.

27 September 2020 3,825 7 View

What is the difference between PCR and the initial steps (before SDS gel) of DNA sequencing? Which method is more effective?

I apologize if my question is not clear. I have a pure chemistry background and I am very new in a Protein Chemistry and Enzymology science.

25 September 2020 8,275 5 View

How to isolate/quantify a fungal enzyme for comparison of control vs. treatment?

I want to compare the amount of an enzyme's expression (laccase for example) or presence in the tissue as a result of the experimental treatment. Is there a protein similar to an antibody that...

31 December 2019 1,557 2 View

How strong is Flag tag binding to Anti-Flag antibody?

I am trying to plan an pulldown assay to find some prey protein for my bait protein. I am interested in using the flag tag because it is small and doesn't disturb structure and doesn't require...

11 December 2019 2,620 3 View

How to calculate the dosage of enzymes in wheat flour?

I have recently started working on improvement of wheat flour for baking. I have come across a research which involved the usage of xylanase, cellulase, beta-glucanase and carbohydrases for...

10 November 2019 3,870 1 View

Can I restrict the amount of one species when using KinTek for kinetic fitting?

I am using KinTek software to do a kinetic fitting. I have determined kinetics of the formations of several species in the time course of 5min and established a model for the enzyme (just as an...

09 October 2019 4,046 4 View

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me. Does somebody have this plasmid?

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me, would be needed for my work. I would like to test the interaction between my labor's new...

05 June 2019 4,351 1 View