The study of the properties and action of enzymes. | Contact experts in Enzymology to get answers
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Questions related to Enzymology
Hello Everyone! I am going to look PPO and POX enzyme activity in fruits. I am getting confuse to make a standard curve. Which reference substances should be taken while making a calibration curve...
07 July 2016 2,290 13 View
Hi there,I am attempting differential scanning fluorimetry (DSF) with my protein and would like to screen it against various ligands to demonstrate binding and stability. I have made a few...
07 July 2016 1,045 4 View
There are two ACP transacylase for converting acetyl-CoA and malonyl-CoA into ACP intermediates. Are there any other ACP transacylase for converting other acyl-CoA into acyl-ACP.
09 May 2016 9,511 3 View
Can anybody provide working protocol for polysome profiling in bacteria ? I have tried protocols given by Methods in Enzymology, Chapter Eight – Analysis of Polysomes from Bacteria,Daoming Qin*,...
04 May 2016 4,557 1 View
I am looking at an enzymes reaction mechanism. I have investigated 2 mutations. both mutations individually reduce the enzymes catalytic activity. The 2 mutations combined render any product...
28 April 2016 6,803 9 View
Hello, I'm trying to determined the Vmax, Km, etc...for a membrane bound short chain dehydrogenase. I am planning on using cell lysates as a source of the enzyme. Do you know of any protocol to...
07 April 2016 1,645 4 View
I measured activity of SOD enzyme with NBT method by Spectrophotometer. SOD (EC 1.15.1.1) activity was determined by measuring its ability to inhibit the photoreduction of nitro bluetetrazolium...
04 April 2016 9,525 22 View
04 April 2016 2,044 5 View
I would like to know if there is an explanation for the increased of the enzymatic activity when the enzyme concentration is decreased. I tried to measure my enzymatic activity by diluting my...
04 April 2016 8,878 7 View
04 April 2016 2,825 10 View
In my assay, the substrate contributes a lot of DMSO, about 3.2% at a 100uM and contributes 0.8% making a total of 4% DMSO concentration in the assay. So we decided to make the DMSO concentration...
31 March 2016 747 5 View
I need the enzyme list to be detailed enough to include the isozymes of a particular enzyme. For example in glycolysis, rather than "hexokinase", the list of enzymes would include "hexokinase-1",...
31 March 2016 478 1 View
Which is the best concentration of DEPC in water solution to inactivate larger amounts of RNases?
19 March 2016 4,405 2 View
What can be the easiest and most reliable method to determine the alpha-glucosidase inhibition mechanism? please comments in detail
16 March 2016 9,194 17 View
Hi All, I want to calculate the L-asparaginase enzyme activity. I ain't sure which formula should I use. If I use the standard one [1 unit equalling the amount of L-asparaginase that liberates 1...
07 March 2016 902 3 View
the method used is Ellman method.
03 March 2016 8,669 1 View
such as, Fundamentals of Enzymology The Cell and Molecular Biology of Catalytic Proteins Third Edition Nicholas C. Price and Lewis Stevens or any other please share with me. thanks
03 March 2016 5,560 6 View
03 March 2016 9,433 2 View
I am aware of the method of determining product inhibition constants and concurrently the "type" of inhibition by comparing the Michaelis Menten graphs generated in the presence of different...
03 March 2016 2,859 3 View
03 March 2016 1,638 18 View
i am trying to conjugate an anticancer enzyme with silver nanoparticles which i intend to prepare using chemical reduction method or by using polysaccharides / polymers as capping/ reducing agents.
17 February 2016 6,506 1 View
Can anyone help me to find the suitable methodology to analyze enzyme activities in N. lugens, S. litura related in insecticide resistant in both of them? i already read some articles about it,...
16 February 2016 8,796 3 View
The number of known enzyme activators is minuscule compared to inhibitors. It may be that finding common features that help binding to allosteric binding sites is difficult due to the variety...
16 February 2016 473 6 View
02 February 2016 935 7 View