The study of the properties and action of enzymes. | Contact experts in Enzymology to get answers
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Questions related to Enzymology
I've already tried by using a comparison of untreated and treated sodium borohydride. Treatment with NaBH4 reduces shiff base to amine, a step that makes trypsine "jump" the catalytic lysine cut...
17 January 2013 3,807 8 View
Our primary experiment results indicated that food resource arabinogalactan protein (AGP) could combine with phenolic compounds, such as epigallocatechin gallate (EGCG), thus can mask bitter or...
16 January 2013 7,420 5 View
I have recently had an experience where, when storing our recombinant proteins long-term in -80, if we flash freeze them in liquid nitrogen they do not precipitate (and if we do not, they...
09 January 2013 9,674 8 View
Adenine phosphoribosyltransferase
01 January 2013 7,125 1 View
01 January 2013 5,662 6 View
I am working with a chlamydial protein that has low solubility. We fusion tagged the protein (with maltose binding protein) to increase solubility and for purification, but when I purify the...
01 January 2013 9,127 18 View
01 January 2013 2,130 9 View
The DNS assay is performed for the enzyme activity measurement.
01 January 2013 7,683 2 View
I'm searching for any information regarding the stability of tween20 (polyoxoethylen (20) sorbitan monolaurate). We employ this detergent as a component of the reaction media in which we study the...
01 January 2013 4,744 16 View
I am working on a chlamydial protein that appears to work in vivo, but once I purified it is not active. I tried testing it with a maltose binding protein to make it soluble, but I fear that it...
01 January 2013 4,317 3 View
I am working on one protein. I need to screen the inhibitors against it. The enzyme activity was known based on the fluorescent reading of the product. As I increased the concentration of...
30 December 2012 8,989 25 View
Due to limited funding for science research, we are committed to make our own organic supplements for microbiological and enzymological research, so please help.
12 December 2012 7,276 3 View
Why does the activity of enzymes decrease as they are diluted in aqueous solutions/mixtures?
12 December 2012 3,156 7 View
Methods in Enzymology
12 December 2012 4,383 5 View
11 December 2012 3,387 4 View
I have encountered this article: http://www.jbc.org/content/276/38/36000.full which is detailed but to me incoherent - I do not understand which material comes when and the order of the procedure...
22 November 2012 8,877 13 View
11 November 2012 1,302 13 View
Normally immobilization is mainly done to improve stabilization and for reuse, how immobilized enzyme separated from an industrial effluent?
11 November 2012 4,384 11 View
To calculate the Km of an enzyme.
11 November 2012 2,328 1 View
GCE modified using carbon nanotubes
11 November 2012 1,558 11 View
I am studying enzymatic transglyosylation of activated glycosyl donors. I submitted several papers, however, I don't really know who pointed out that nitrophenyl glycoside is one of the suitable...
11 November 2012 8,274 2 View
06 November 2012 5,746 1 View
06 November 2012 7,753 2 View
I am working on thermostable superoxide dismutase (SOD) and isolated SOD from few Plant sp. which are resistant to heat denaturation at high temperatures of 80 °C and above. There are reports on...
25 October 2012 3,757 3 View