The study of the properties and action of enzymes. | Contact experts in Enzymology to get answers
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Questions related to Enzymology
We are working on optimization of enzymes from bacteria. So I need to design a model with the parameters of pH, temperature, substrate concentration and fermentation time. Kindly help me with an...
12 December 2013 3,071 1 View
In Science Magazine this week, X. Xie wrote a short piece, on how the MM equation holds at the single-molecule level: ⇢ http://bernstein.harvard.edu/papers/Science-2013-Xie-1457-9.pdf One of...
12 December 2013 5,279 0 View
I want to measure MnP activity by using the methodology described by Kuwahara and I need the molar decadic absorption coefficient for phenol red. How can i find this coefficient?
12 December 2013 4,885 1 View
I'm trying to make zymograms to evaluate endoglucanase activity, but had no luck. I have tried several protocols using a 0.2 IU per well in gels 1 mm SDS-PAGE and Native containing CMC, incubating...
11 November 2013 8,033 2 View
Generally enzyme unit is defined as, "amount of enzyme required to release μM of product per min or require to catalyze uM of substrate per min." But I am using 50 ul of purified enzyme (0.01 μM...
11 November 2013 9,742 9 View
01 November 2013 9,656 8 View
I am optimizing pH for my cellulase enzyme with substrate CMC and pNPG. Can anyone provide me with a good protocol for pH optimization?
10 October 2013 3,355 6 View
Are both of them in humans?
10 October 2013 3,621 1 View
What does this statement mean - The uptake of substrate at a specific concentration may reflect both Km or Vmax or both. If Km of a transporter is independent of one of its regulator then the...
10 October 2013 612 7 View
I am interested in the procedure for my research work.
09 September 2013 6,019 3 View
I am working on an extracellular protein. After taking supernatant I performed protein assay and good activity of protein was observed but no band was observed when loaded on SDS PAGE.
08 August 2013 7,368 23 View
We estimate the FAD saturation of purified an acyl-CoA dehydrogenase homologue by the ratio of the absorption maxima in the range of 268nm, 366nm and 449nm (ratio of 5:0.7:1). We consider all FAD...
08 August 2013 5,000 3 View
Could someone tell me how to adjust the pH of Arginine to 9.8? If possible please suggest some papers on arginase assay with crude cell lysates after some drug treatments.
08 August 2013 7,419 0 View
I am using activated calf thymus DNA (GE healthcare) that is treated briefly with DNaseI. Unfortunately the new lots we bought from GE are over-digested and I don't get any activity of the T4 DNA...
08 August 2013 1,389 0 View
Is there positive or negative correlation?
07 July 2013 4,608 12 View
To investigate the acute toxicity of cadmium and lead on larvae of two Chironomid species, different larval instars (first-fourth) were exposed. Now if I want to study about enzymes involved in...
07 July 2013 5,610 1 View
Does anyone know some reliable protocol for extraction/isolation of NAD(P)H and NAD(P) from bacterial cells (G-)?
06 June 2013 5,636 0 View
The protein of interest is a glycoprotein and the 3D structure is not resolved yet.
06 June 2013 7,870 0 View
After assaying a number of compounds for inhibitory activity, I am now using GraphPad to globally fit nonlinear regression curves and determine the mechanism of action. It seems that two of the...
06 June 2013 2,747 12 View
I am working on some DNA aptamers and interested to evaluate the inhibition potency and mode of inhibition of this aptamers against particular target enzyme. Briefly, I would like to know a good...
06 June 2013 7,276 12 View
We are studying the kinetics of a microbial enzyme, and have run across an interesting phenomenon I have not observed before. In attempting to establish a Vmax and Km for the enzyme by varying...
06 June 2013 6,089 5 View
I want to study the kinetics and thermodynamics properties of cloned, purified alpha amylase. It is active at 90°C. Can anyone suggest a good experimental guide line to set up the experiment to...
06 June 2013 5,892 2 View
Lipase catalyzed bio diesel production...
06 June 2013 3,418 1 View
For example after running a column chromatography I realized that five of the collected fractions contained high enzyme activity (after running an assay on each). So I've pooled the five fractions...
05 May 2013 4,688 8 View