The study of the properties and action of enzymes. | Contact experts in Enzymology to get answers
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Questions related to Enzymology
My graph for standard curve doesn't touch at zero. I always get a graph straight but touching higher at about 2 parts. I am using the lowery method and the procedure is like this:2% Na2co3...
02 February 2015 2,539 9 View
Kindly tell me the well-known enzyme inhibitors for the following enzymes: Adenylosuccinate lyase Dihydroorotate dehydrogenase N- myrsitoyl transferase Pteridine reductase 1 Thank you.
02 February 2015 2,849 2 View
hello thr, I am trying to purify my enzyme by using IEC (anion) using biorad FPLC unit. I tried isocratic as well as gradient elution methods but getting four flat peaks out of which first two are...
02 February 2015 6,491 4 View
Dear Colleagues..I would like to invite you to contribute a book chapter entitled "Forestry waste: Potential feed stock for enzyme production, In edited book “Agro-industrial wastes as feed stock...
02 February 2015 406 0 View
We tried a high temperature(100 C 1h)but doesn't work
30 January 2015 8,805 9 View
I would like to find an easiest way to detection serine protease activity in Enterococcus faecalis. Thanks.
18 January 2015 6,765 4 View
01 January 2015 2,134 10 View
01 January 2015 8,267 5 View
Advances in Dairy Research is the field that deals with the Biochemistry, Physiology, Cell biology and endocrinology of lactation, animal science, milk production and composition, Biotechnology...
01 January 2015 8,698 21 View
I'm able to detect phenotypic differences among mutant strains lacking one or two catalases, but when I try to detect individual catalase activities by zymography after native PAGE, I'm only...
26 December 2014 8,986 3 View
Hello Every One, I have taken UV spec readings (for 3 minutes) for the activity of Xylosereductase and xylose dehydrogenase by the method defined elsewhere by using NADPH/ NAD. from that...
12 December 2014 3,919 3 View
12 December 2014 9,906 4 View
Hi, I am about to carry out a few enzyme assays. After protein purification and dialysis to remove the salts, what is the acceptable maximum time to keep the protein at 4 degree before performing...
25 November 2014 4,656 10 View
Catalase activity was measured in a reaction mixture (1 ml) containing 100 mM Na2HPO4 buffer pH 7.4, 10mM H2O2, 20ug protein extract. I took reading at od240 every 5 sec. got readings 0.47, 0.402,...
24 November 2014 2,859 4 View
Please elaborate the change in Enzyme kinetics (v, Km etc) between an immobilized and an enzyme in solution. Thanks and regards Jitendra
19 November 2014 7,533 14 View
I having a problem regarding the formula and calculation of catalase activity through spectrophotometry (Aebi,1974)
11 November 2014 8,549 10 View
11 November 2014 4,411 5 View
11 November 2014 3,646 15 View
I need a very good protocol for competition binding assay. I have a complex and a third protein. I expect the third protein to dissociate the complex by binding with one partner of the complex....
11 November 2014 6,134 3 View
Does anyone have complete assay protocols with formulas for Catalase (EC 1.11.1.6), SOD (EC 1.15.1.1), Glutathione Peroxidase (EC 1.11.1.9), Glutathione-S-transferase (EC 2.5.1.18)? As methodology...
09 September 2014 9,934 4 View
08 September 2014 8,066 3 View
I am looking for an enzyme that can hydrolyse a fucoidan polysaccharide into oligosaccharide units.
28 August 2014 375 1 View
The cofactor-free enzyme is the enzyme without cofactor?
22 August 2014 7,779 7 View
How does one quantify total cellulose, hemicellulose, and lignin in biomass before pre-treatment of lignocellulosic biomass?
18 August 2014 3,072 22 View