How can a 'Protein Chemist' coordinate with an 'Enzymologist'?

More Sanjay Mishra's questions See All

Should we treat 'Aging' as a disease?

Medical Professionals, Biochemists, Molecular Biologists

06 July 2016 1,002 0 View

How can I assay phosphorylase a and b spectrofluorometrically?

I am looking forward to monitor a reaction of conversion of phosphorylase b to a and vice versa.

05 June 2015 4,724 0 View

Similar to macromolecules like carbohydrates, protein and lipids, is it possible to identify an enzyme using a single step protocol?

If the source is known and we are interested in modifying the source quality by altering the enzymatic modulation.

04 May 2014 8,071 3 View

What are the major biochemical and biophysical techniques to isolate and purify a multienzyme complex?

Pyruvate dehydrogenase complex.

03 April 2013 4,290 0 View

As far as my experience is concerned, soluble enzymes are rather easier to immobilize. How can this be easily applicable to membrane bound enzymes?

Google

01 February 2013 705 0 View

How can team spirit be helpful in upgrading the level of research output?

31 December 2012 2,199 4 View

How can the knowledge of biochemistry be helpful in explaining and propagating 'Bioprocess and Fermentation Technology'?

31 December 2012 2,511 4 View

Do data obtained from X-Ray Crystallography explore the studies concerning with molecular dynamics using computational biology ?

11 December 2012 3,006 8 View

Which one out of 'Impact Factor' and 'SCI' is better to explain the worth of a 'Research Paper'?

Google

11 December 2012 7,767 6 View

Why does the activity of enzymes decrease as they are diluted in aqueous solutions/mixtures?

11 December 2012 2,929 7 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View