How can a 'Protein Chemist' coordinate with an 'Enzymologist'?

More Sanjay Mishra's questions See All

Should we treat 'Aging' as a disease?

Medical Professionals, Biochemists, Molecular Biologists

06 July 2016 1,239 0 View

How can I assay phosphorylase a and b spectrofluorometrically?

I am looking forward to monitor a reaction of conversion of phosphorylase b to a and vice versa.

05 June 2015 4,843 0 View

Similar to macromolecules like carbohydrates, protein and lipids, is it possible to identify an enzyme using a single step protocol?

If the source is known and we are interested in modifying the source quality by altering the enzymatic modulation.

04 May 2014 8,191 3 View

What are the major biochemical and biophysical techniques to isolate and purify a multienzyme complex?

Pyruvate dehydrogenase complex.

03 April 2013 4,428 0 View

As far as my experience is concerned, soluble enzymes are rather easier to immobilize. How can this be easily applicable to membrane bound enzymes?

Google

01 February 2013 870 0 View

How can team spirit be helpful in upgrading the level of research output?

31 December 2012 2,326 4 View

How can the knowledge of biochemistry be helpful in explaining and propagating 'Bioprocess and Fermentation Technology'?

31 December 2012 2,644 4 View

Do data obtained from X-Ray Crystallography explore the studies concerning with molecular dynamics using computational biology ?

11 December 2012 3,127 8 View

Why does the activity of enzymes decrease as they are diluted in aqueous solutions/mixtures?

11 December 2012 3,130 7 View

Which technique may be the best among (a) Radiometric assay of an enzyme; (b) HPLC assay of an enzyme; (c) Spectrofluorimetric assay of an enzyme?

Methods in Enzymology

11 December 2012 4,358 5 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View