Can I use ultrafiltration pipe without ammonium sulfate precipitation in protein purification process?

After cell disruption, centrifugation, if the supernatant was not treated by ammonium sulfate precipitation or dialysising against buffer, can I use ultrafiltration pipe directly to concentrate...

05 June 2013 6,978 2 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View

In Michaelis-Menten Kinetics, why K(subscript)On have units (mol^-1)(time^-1)?

where K(subscript)On is the rate of Enzyme (E) + Substrate (S) going to Enzyme-Substrate complex (ES). I think I understand why K(subscript)Off and K(subscript)catalysis have units of time^-1....

11 January 2021 649 8 View

Should I normalise data when measuring fluorescence intensity ?

I'm measuring fluorescence intensity of the product in an enzyme reaction in 37 celsius degree. I set a kinetic measurement in 1 hour. The fluorescence intensity of the BLANK ( contains only...

03 January 2021 5,554 5 View

How to calculate enzyme's energy of activation without rate constant (+excel)?

I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...

25 December 2020 8,738 3 View

Why nitrocefin is preferred over CENTA?

The chromogenic beta-lactamase substrate CENTA is at least five times cheaper than the other famous substrate nitrocefin and can be used with most enzymes, still, nitrocefin is preferred. Any...

17 December 2020 3,735 2 View

How much time can we conserve enzymatic extract?

Hi every one, I am extracting enzymatic extract from plant (50 mM Sodium phosphate buffer (pH 7.8), 0.1 mM EDTA, 0.1 % (v/v) Triton; 1 mM PMSF). I mesure the protein content and enzymatic...

10 December 2020 7,759 2 View

Enzym Decantation or Filtration?

Hello, I am looking for an efficient method to separate My Enzyme from my reaction mixture. I tried filtration many times but I am Never able to fully separate the enzyme from the reactants. If I...

07 December 2020 8,865 2 View