Determination of the activity of some Exoenzymes produced by Vibrio cholerae such as Protease, Elastase, Lipase, Lecithinase and Hemolysin?
Some microorganisms can produce enzymes such as lipase, elastase, protease. So how can we detect the activity of these enzymes in case they have been presented in our petri dish?
31 December 2012 1,385 15 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?
17 July 2024 935 1 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...
13 July 2024 2,255 3 View
I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...
04 July 2024 8,100 4 View
I am conducting research on the antimicrobial synergism activity of lactobacillus strain with B.subtilis. in a recent experiment, we observed inhibition of B.subtilis growth with high...
01 June 2024 338 2 View
I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...
31 May 2024 6,444 1 View
Protein content in malt processing can have several effects, primarily related to its impact on the enzymatic activity during mashing and its influence on the final characteristics of the malt and...
29 May 2024 4,074 2 View