Asiaticoside does not have week O-H or C-H bonds and does not quickly reacts with radicals.

Yurii V Geletii Sir ! can you please suggest any important ways that can i follow. Thanks in advance

Benish Nawaz The herbal bioactive compound asiaticoside may not be a strong direct free radical scavenger in the DPPH assay.

Weak to moderate DPPH scavenging activity has been reported for asiaticoside in various studies. Compared to standard antioxidants like ascorbic acid or quercetin, asiaticoside exhibits lower radical scavenging capacity. Asiaticoside lacks strong hydrogen-donating functional groups like flavonoids, which may explain its lower direct DPPH activity.

It may be exerting antioxidant effects through indirect pathways by enhancing the body's antioxidant defense systems, possibly due to its ability to stimulate endogenous antioxidant defense mechanisms (e.g., increasing SOD, catalase, and glutathione levels).

But higher drug concentrations may enhance scavenging ability.

Further research on its nanoformulations or synergistic combinations with potent antioxidants could improve its potential.

See the answer of Athira V. Anand

Athira V. Anand Many thanks

Benish Nawaz I'm glad that you found the information useful. If you have any further insights or questions, feel free to share

Athira V. Anand could you please mention the concentration of drug how much i can use for Antioxidant studies because i have tried higher but still i did not get appropriate results ...please just mention... it will be helpful for me ....thanks in advance