I have run a sample in ESI-(+)-MS/MS mode. Can we get an adduct like [M+H+Na] in the spectra. Also can you help me with how we can better identify and make sure this is our compound in the LC-MS spectra.
"Na" is everywhere, esp. on glass so is commonly observed as an adduct in ESI.
If you have not done so already, please run a known std of your compound on the exact same system using the exact same method. This data will be useful during interpretation. LC-MS data is only as good as the method used to acquire it (and sometimes the method parameters are not valid). You will need to provide the full analysis method used (HPLC-MS) and chromatograms (showing all scales) with ion data and settings to a local expert familiar with your sample type(s) and application/industry. Perhaps your school can suggest one available for hire in your area?
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