How can we optimize reaction conditions (pH, temperature, time, concentration) systematically for different biological systems ?

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Maryam Juma Yaseen

Consider system-specific factors (e.g. enzyme thermal stability, cell viability, substrate inhibition as well as determining the critical PH and temperature of the biological medium you are working with, which is more influential than time, meaning it is essential to know the aspects of each biological system you are working on

Youssif Elzawiei

There are many factors like pressure, type of material, and ambient conditions.