In my lab, we were preparing DNS reagent to measure reducing sugars after amylase incubation. That reagent requires addition of potassium sodium tartrate (300 g for every 1000 mL of reagent) with NaOH 1 M. Problem is, the tartrate is not dissolving even after prolonged heating over 100 C avoiding evaporation. We also tried adding it little by little but after certain spot dilution stopped.
Only thing I could think is that the tartrate was not in good conditions and that’s why it didn’t work out. But honestly, I’m not sure.
Has anyone hear experienced something similar and could share any suggestions? Thanks to all of you.
Dear Anndrea,
I suppose the DNS reagent is saturated with respect to Na-K- tartrate. So the undissolved salts shouldn't be any problem.
The reagent is prepared using sodium hydroxide 2M in 100ml and potassium and sodium tartrate salt 150g and diluted in a litre of water provided the reagent is kept in a dark glass bottle to avoid the effects of light on the reagent.
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