I am trying to run a sulfatase activity assay using purified steroid sulfatase enzyme and 4-MUS as a substrate. The protocol I am following says to use 0.6 units of enzyme however the purified enzyme I have purchased did not come with a specified enzymatic activity (units). I'm not sure if this is a known figure or if I have to calculate this? any help is appreciated.
Adam B Shapiro
In the absence of sufficient information on the specific activity of the protein you purchased, you could do a simple experiment in which you measure the product formation at various enzyme concentrations to find the concentration that gives you the activity you need.
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