Perhaps because there is very little substrate - Glucose is present at 5 mM, about 900 ppm or

Hi, Dr. Phil Geis. I was actually trying to mimic candidemia conditions. Now I realize that, besides basic nutrients, there are additional host factors (such as growth factors, proteins, and cell interactions) that allow Candida to survive and adapt in the bloodstream. This was just a preliminary test. I’m well aware of the high cost of Human Plasma-Like Medium. For my standard experiments, I usually grow Candida in RPMI, as recommended by EUCAST. Thank you for your answer!

You may be more apt to induce morphogenesis than growth

https://microbeonline.com/germ-tube-test-candida-principle-procedure-results-interpretation/

Yes, that makes sense. I truly appreciate your assistance Phil Geis , thank you.