Dear colleagues, I would like to ask you if you have any experience how to use data from ELISA analysis when my standard curve is not as showed in the manual? I have many standard curves that have lower values, below 1,0 when using OD 450 nm measurement. Can I use these results for future evaluation? Thank you.
The OD values are variing according to environmental conditions, mainly the room temperature. Lower ODs are usually no problem, if let's say, OD is not below 0.1
This is the reason, why the calibration curves have to be performed always in parallel to your measurements and why you should never use a calibration from another day.
Sure, you can also use the obtained results, since they are related/based to/on the same (not optimal) standard curve! Thus, the results are (isolated in this experiment) obtained under the same (maybe not optimal) conditions and are therefore meaningful in this context/setting.
However, it may be somewhat difficult to compare these results with other findings under different (maybe more optimal) preconditions.
If you do not need the actual quantity (eg ng/mL), you can easily used directly OD value as percentage or relative ratios
The OD values are variing according to environmental conditions, mainly the room temperature. Lower ODs are usually no problem, if let's say, OD is not below 0.1
This is the reason, why the calibration curves have to be performed always in parallel to your measurements and why you should never use a calibration from another day.
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