currently we are working on various analgesics and anti-inflmmatory drugs for mechanistic evaluation. In this regard we are looking for procedure to evaluate PGE2 from paw (carragenan method), tibia anterior and gastricnemus muscle of rat.
The liquid extraction technique can be used with liquid solvent to dissolve with each other. The layers are then separated, the organic layer is taken and the spectral absorption is measured. However, after calculating the maximum wavelength of PGE and by the Per Lambert Act, the concentration is measured and the amount of material to be measured think you for question me
Fatimah Alghurabi
Thank you for your answer. But i am looking for procedure, hw to extract it from rat paw / muscle and which type of solvents / buffers to be used for extraction.
Pharmacological characterization of carrageenan induced heat muscle
hyperalgesia in rats using non-selective, preferential and selective
COX-2 inhibitors. - Atul R. Chopade a,c,*, Fahim J. Sayyad a, Nilofar S. Naikwade b
The above article has cited the below two articles for isolation of PGE2, in which one article is not having any info that what i expected and other article is in japanese. Can any body help me in translating from Japanese article?
Charles AW, Edwin AR, George WN. Carrageenin induced edema in hind paw of
the rat as an assay for anti-inflammatory drugs. Proc Soc Biol Med 1962;
1:544–7.
Matsuda R, Tanihata S. Suppressive effect of sialic acid on the prostaglandin
E2-mediated oedema in carrageenin induced inflammation of rat hind paws.
Nippon Yakurigaku Zasshi 1992;99:363–72 [in Japanese].
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