I'm going to perform an ELISA for fish GnRH (using CUSABIO). The "Technical Tips" pamphlet that came with the instructions manual says I need to centrifuge all reagents before use "as to concentrate them to the bottom of the tube" but does not specify at what speed or for how long. I cannot find this information anywhere in the manual or online.

Does anyone know what speed and for how long I should centrifuge the reagents?

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