Dear Sahil,
The following two methods are used to calculate the grain size from a SEM image or from microscope optical image:
(1) Image software for grain size analysis and measurement ...
Video for calculate the grain size from a SEM image or from microscope optical image▶ 2:54
https://www.youtube.com/watch?v=gVlcRc9JczU
Apr 21, 2014 - Uploaded by Divergent Rays Computing Inc.
Grain size analysis in microscopy is useful for inspection and quality control ... How to measure nanoparticle.
For viewing the you tube, please use the following linke:
https://www.youtube.com/watch?v=gVlcRc9JczU
The intercept technique:
(2) Draw a random straight line through the micrograph and count the number of grain boundaries intersecting the line. The average grain size is calculated by the division of the number of intersections by the actual line length.
Grain size average =1/number of intersections/actual length of the line.
Actual line length = Measured length/ Magnification.
Hoping this will be helpful,
Rafik
Dear Sahil,
The following two methods are used to calculate the grain size from a SEM image or from microscope optical image:
(1) Image software for grain size analysis and measurement ...
Video for calculate the grain size from a SEM image or from microscope optical image▶ 2:54
https://www.youtube.com/watch?v=gVlcRc9JczU
Apr 21, 2014 - Uploaded by Divergent Rays Computing Inc.
Grain size analysis in microscopy is useful for inspection and quality control ... How to measure nanoparticle.
For viewing the you tube, please use the following linke:
https://www.youtube.com/watch?v=gVlcRc9JczU
The intercept technique:
(2) Draw a random straight line through the micrograph and count the number of grain boundaries intersecting the line. The average grain size is calculated by the division of the number of intersections by the actual line length.
Grain size average =1/number of intersections/actual length of the line.
Actual line length = Measured length/ Magnification.
Hoping this will be helpful,
Rafik
Free software (ImageJ): http://imagej.nih.gov/ij/
Your specimens are not good for measurments. You do not see grains. You need to change etching (and/or polishing) technique.
I agree with Vladimir that measuring grain size from your SEM images will be difficult as cannot see particles.
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