How do you edit a m2p file to get nice Dssp images?

I am using the m2p file from https://ringo.ams.stonybrook.edu/index.php/MD_Simulation:_Protein_in_Water_(Pt._2) but my ss.jpg is looking pretty sad I am converting via imagemagick convert ss.eps...

17 January 2021 498 3 View

How to script VMD tcl within bash script?

Greetings, I want to run a vmd script within my bash script. I am using the text only version of VMD for linux for my windows terminal ubuntu subsystem and I would like to generate code within my...

16 January 2021 192 5 View

What if the informal business in a particular country are forced to be registed and are subject to tax obligation?

What will be the effect on the daily living for an average citizen of that particular country? Taking into consideration the contribution of informal business being one of the affordable option...

06 June 2018 5,261 4 View

Sciex Triple TOF systems, can you comment on calibration and mass stability issues?

Hi I have money to buy one of these for both metabolomics and proteomics. How stable are these instruments especially the 5600 model as regards mass accuracy? Thanks Brendan

17 October 2017 1,097 3 View

Hi, does anybody have any tips for immunostaining of cartilage samples?

Hi, does anybody have any tips for immunostaining of cartilage samples? I seem to be getting a high rate of sample detachment from slides. I am using an autostainer to immunostain cartilage...

23 August 2016 978 2 View

What statistical test should I use?

18 July 2016 8,132 4 View

How do you solve a root rot (pythium) problem in a floating raft aquaponic system?

Working at an farm in the Dominican Republic that has a commercial scale floating raft aquaponic system. The system is poorly maintained and water quality parameters are scarcely recorded. The...

29 January 2016 4,312 8 View

Does anyone use the iBlot gel transfer system?

06 October 2015 6,098 5 View

What statistical test would be used for this occurrence setup if possible?

Suppose I had 4 treatments done to mice to determine when normal bowel movement would occur faster or as near to treatment (a).a) no treatment, negative controlb) antibiotics onlyc) antibiotics...

27 March 2015 1,394 2 View

Is there a way to assess quality of stool for mice across 4 treatment groups without looking at the bacterial content?

Treatments: 1. Normal (negative control)2. Antibiotic3. Antibiotic followed by intervention A4. Antibiotic followed by intervention BThe main objective is to compare A and B.

25 March 2015 4,722 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View