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I'd like to measure trans fatty acids in industrial foods. What method of analysis would you recommend? I've heard of microwave-assisted extraction techniques for processing samples. My lab only...
01 May 2024 5,427 1 View
Please ignore varietal difference and make your argument general.
30 April 2024 6,721 1 View
This is specific to NON-DIABETIC low blood sugar. It seems that the one book outlines “flat curve hypoglycemia” very well with a specific graph, yet finding current research with journal articles...
30 April 2024 8,497 2 View
I am trying to detect organic acids on my waters LC, which is equipped with a W410 refractor index detector. I am using a Aminex HPX-87H Column which is supposed to work to separate organic acids....
27 April 2024 3,174 1 View
Respected! I am new to using Ansys fluent. I am combustion and use heat of combustion to boil water as shown in figure 2. I have achieved combustion using species transport model. But my problem...
26 April 2024 5,318 0 View
I need to prepare EPPS 200 mM with 5 mM DTPA. Is DTPA soluble in water or do I need to adjust pH first? Thank you
26 April 2024 808 1 View
I made some solid dispersion samples with VAL (valsartan) as the active compound. I was expecting it to become amorphous form. However, when I first run it through DSC, the pure VAL drug has some...
26 April 2024 2,942 4 View
I am working on isolating Naive CD4+ T cells from human PBMCs using the Miltenyi AutoMacs. However, I am obtaining a higher percentage of Naive CD4+ T cells from the PBMCs than expected. I would...
26 April 2024 4,775 1 View
When Hematoxylin - Eosin staining is performed in mice tumors, which are the most important tumor tissue characteristics that determine the existence of necrotic zones? What changes in tumor...
25 April 2024 3,786 1 View
How does evidence-based medicine help clinicians make informed decisions about patient care?Understanding Evidence Levels in Evidence-Based Medicine: A ...
25 April 2024 5,126 1 View
I possess data on JV, current, voltage, and current density obtained from a simple diode (metal-semiconductor). Currently, I aim to plot the Richardson curve and Arrhenius plot to determine the...
25 April 2024 5,520 6 View
I have done formulation development of tablet for which i have considered two independent variable. Results are plotted in a statistical analysis software and polynomial equations are generated....
25 April 2024 4,098 1 View
I have animal feed sample. I am using 1 g feed and 5 ml buffer. After protein extraction. I diluted my sample 20 and 40 times. but the concentration and Percentage of protein varies in both...
24 April 2024 4,755 1 View
I wanted to calculate the activation energy of IgG from Tm and varying scan rates obtained from DSC. I have read some papers and they have used lumry erying model. But what I have known is that is...
22 April 2024 4,780 4 View
Are there effective methods for creating different concentrations of a liquid plant sample, particularly if it has a high water content? Can lyophilization or freeze drying be utilized for this...
21 April 2024 5,803 0 View
How can social enterprises effectively measure and communicate their social impact?
20 April 2024 2,904 0 View
I'm currently analyzing the intestine using villin CRE knockout mice. While my team specializes in liver research and we're expanding into intestinal studies, we're not very familiar with...
19 April 2024 3,900 1 View
I tried glycine buffer but the yeast cells acidify the buffer so the pH goes down to 7-6 overnight. I need something that will stay around 9 and that isn't toxic to the cells.
19 April 2024 8,120 4 View
I directly cloned cry1A gene p234 expression vector and transformed E.coli Bl21 strain. but expression protein cannot appear.
19 April 2024 5,902 3 View
I made a buffer solution dissolving 79 g of NaH2PO4.2H2O in 1000 mL DI water. Then tried to adjust the solution pH to 6.0 using HCl or NaOH. Initial pH was below 6, so I added NaOH into the...
19 April 2024 6,271 1 View
The non-linear response of the electron in the driving laser field, we always mention in solids, inter-band polarization, and coherent intra-band dynamics of the electron. I want to deepen my...
18 April 2024 3,548 1 View
i seeded CACO2 on 0,4 um PET transwell insert, i want to see my cell layer in transversal view in order to monitor the polarization and formation of brush border. To do this, i fixed with 4% PFA...
17 April 2024 6,646 1 View
microwave
17 April 2024 10,183 4 View
Due to factors outside of my control, peptides in my ESI-MS data have been ionised normally by protons ([M+2H], [M+3H]...) but also by sodium ([M+2Na], [M+3Na]...). Is there a way to configure...
17 April 2024 10,144 2 View