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The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65...
15 July 2024 1,638 0 View
I am working on powder metallurgy, and during the compaction process, the powder does not pelletize and remains in its powder form. To address this issue, I am considering using binders to improve...
14 July 2024 9,226 2 View
TPA is a cornerstone in health insurance as every insurer after enrolling in a policy is waiting to get the benefit out of it, a human nature. because of existing norms, there is a gap between...
13 July 2024 7,550 0 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
13 July 2024 1,186 0 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
13 July 2024 6,536 0 View
Hello! Have you ever observed that when measuring the viability of freshly thawed PBMC with trypan blue, the value is higher than with Annexin/PI staining or some other staining that takes into...
11 July 2024 776 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
11 July 2024 5,063 2 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
11 July 2024 9,335 1 View
Hi, Does anyone have experience and success of isolating, culturing and stimulating hamster splenocytes for T-cell analysis (FACS and ELISPOT)? I am currently using a protocol that we routinely...
10 July 2024 6,418 1 View
Recently I looked up a literature《A Glucose-6-Phosphate Isomerase Peptide Induces T and BCell-Dependent Chronic Arthritis in C57BL/10 Mice Arthritis without Reactive Oxygen Species and...
10 July 2024 6,770 0 View
Lorsque nous nous posons cette question , nous nous basons sur un fait et constat très simple : Dans les manuels d’apprentissage du chinois, qui tiennent lieu de programme pour l’instant dans les...
09 July 2024 7,892 3 View
Hi, I am trying to model laser cladding in Ansys FLUENT. There are two phase: one is base metal surrounded by an inert gas (shown in the image). I want to add a cladding layer of the same metal....
08 July 2024 1,324 8 View
Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...
08 July 2024 8,398 2 View
is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?
06 July 2024 4,381 3 View
How is pharmacogenomics transforming personalized medicine, and what are the current challenges in its implementation?
06 July 2024 5,370 2 View
I want to detect Chlamydia psittaci bacteria in birds' feces by PCR.
06 July 2024 3,901 1 View
Good morning. I would like to understand how the (possible optimal) route of transmission lines is determined. Likewise, the number (and type) of structures. Could you please recommend good...
05 July 2024 6,952 0 View
Solid state NMR study
04 July 2024 598 1 View
Hi! I am working on a biophotonics project in which I need isolated but inactive CD4+ splenocytes. Every protocol and piece of literature I have found immediately activates and cultures...
03 July 2024 5,003 0 View
"Today several adjectival phrases have been used to describe English like ‘International Language’, ‘Lingua-Franca’, ‘Language for Globally Connecting’, ‘Library Language’, ‘Official Language’,...
02 July 2024 8,610 5 View
I'm currently working on gel purification in order to get DNA fragment for T4 ligation. But I have been able to take only 8.5ng/μl as a highest concentration of DNA fragment. And could not get...
02 July 2024 5,044 9 View
I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...
02 July 2024 7,159 1 View
Hi all, I am looking for some syntethised samples of mixed-layer clays to perform infrared reflectance spectroscopy measurements in the VNIR-SWIR-MWIR-LWIR regions. Best regards, Lionel Fonteneau
02 July 2024 3,937 2 View
Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...
30 June 2024 9,816 1 View