Drugs

Contact experts in Drugs to get answers

1,486 views 2,938 posts

Questions related to Drugs

Is it possible to do classroom action research for only one cycle?

The classroom Action Research (CAR) method is derived from the root action research. Because it occurs in the classroom frame, it is called CAR.

16 March 2022 9,233 3 View

How many Endothelial cells are needed for MTS assay?

Hello! I was wondering how many Human Endothelial Cells/well are usually used for MTS assay using Human Endothelial Cells! Thank you for your time!

16 March 2022 1,166 2 View

How to determine the MIC of silver nanoparticles?

I have prepared the silver nanoparticles via chemical reduction method using NaBH4. Upon turning yellow color of the solution I stopped the reaction. I got UV vis peak of nanoparticle solution at...

15 March 2022 7,442 14 View

How to quantitate protein in conditioned supernatant?

I am trying to purify some secreted protein in culture medium containing 10% FBS. But I need to know what is the amount of protein in the medium to do this. I will be using the Bradford Assay for...

14 March 2022 2,212 8 View

What are the types of target validation techniques in drug discovey?

Hi, I am new to target validation (a student studying this) and want to know what kind of techniques are there for target validation? I want to target a protein for example in x disease and this...

13 March 2022 6,442 2 View

Can anyone recommend the best protocol for Casein agar plate assay ?

I have isolated a plant protease, for which I have to perform Casein agar plate assay. I am unable to sort out the following trouble faced during the preparation of the same. Preparation protocol...

10 March 2022 5,944 5 View

Can we use c6 cells in undifferetiated form as an oxidative stress model?

I would like to make an oxidative stress model for studying the antioxidant activity of a drug using C6 cells. Can I use the undifferentiated cells for the same.

09 March 2022 1,011 5 View

Protocol for SUV formation from MLV vesicles?

Dear all, Could someone share/suggest a good protocol for preparing small unilamellar vesicles (SUVs) from multilamellar vesicles. Without using an Extruder. Thanks in advance

08 March 2022 5,077 5 View

Protocol for in vitro melanin content measuring assay and free radical scavenging assays?

Need help regarding protocols

08 March 2022 2,226 3 View

What are the causes and Prevention methods of Diabetes mellitus?

Please give a simple brief summary

07 March 2022 1,537 16 View

How to prepare Liposome for strong acidic drugs having pKa - 2.9 & Highly Soluble. Which kind of lipids & method of preparation is best suited for it?

General Properties of molecules: Molecular weight- 4000-6000 dalton. pKa - Strongest acidic: -2.9 Strongeest Basic: -3.6 log P: -8 Polymorph - Amorphous in nature Sterioisomer -...

02 March 2022 2,966 2 View

How to Compare the safety of two drugs?

I have two chemotherapy drug (A and B), at baseline, after first cycle, second cycle and third cycle, I want to compare between two drugs after each cycle and and which drug associated with more...

02 March 2022 8,475 1 View

How to find different expressed genes regarding to tumor metastasis from clinic ?

I have ready collected several transcription data of tissues from patient(almost have metastatic region) with typical cancer . Is it OK for me to only compare the original cancer site v.s....

02 March 2022 4,809 2 View

How to analyze myoglobin value in fresh ground meat mixed with red dye using UV spectrophotometer?

Dear all., I'm analyzing myoglobin content in ground fresh meat that has been mixed with red pigment solution using UV spectrophotometer at 503, 525, 557, 572, 582, and 700 nm. My red pigment has...

28 February 2022 9,624 11 View

Is it fine if I combine introduction with literature review? How does it feel to readers?

Sometimes you find it necessary to include literature/cite work to firmly assemble your introduction, and too, thrash some disagreements, and differences to give your introduction a sense of...

27 February 2022 4,941 23 View

How the C.elegans get drug from NGM agar plate?

I’m doing drug assay on C. elegans, I’m adding drug on NGM agar media how C.elegans will get drug from the agar plate Still question in my mind looking forward for a appropriate answer and...

26 February 2022 4,771 2 View

What important considerations should be kept in mind before embarking on Phacoemulsification of lenticonus?

The cases of lenticonus operated by me had two common problems 1. Lenses were extremely hard so I had to resort to maximum phaco power. 2. Lenses had least ligamentous support.

25 February 2022 8,998 0 View

Can anyone guide us with a workflow on how to do 2D QSAR analysis?

We are a group of Master's degree students, majoring in Microbiology. We were introduced to Computational biology and were very interested in the subject. We decided to do a project on the QSAR...

21 February 2022 8,295 3 View

Can Agrobacterium rhizogenes play as a elicitor role?

I have not induced successfully hairy root by using Agrobacterium rhizogenes in monocotyledons. I wonder that whether plant can be influenced on secondary metabolism by unsuccessful tranformation...

21 February 2022 5,986 6 View

How to analyze LC-MS chromatogram or simply identify compounds from crude plant extracts by LC-MS chromatogram. Any software that could be helpful?

I have done LC-MS analysis of crude plant samples. Now I need to identify compounds from the LC-MS chromatogram. Need your help to identify compounds.

20 February 2022 4,807 5 View

During liposome preparation with hydrophilic drug, liposome get precipitated. Why is this happening,&does anyone have any suggestions about it?

Preparation of liposome is carried out by thin film hydration method with cholesterol, chloroform and phosphatidyl choline. During the process of film hydration drug is introduced in to the...

18 February 2022 1,919 3 View

What is the best way to determine whether all or vast majority of single gene mutations leading to a disease is due to loss-of-function mutations?

For instance, RPE65 mutations can be missense frameshift etc, but is there an efficient process to know whether the vast majority of mutations lead to a functional loss?

18 February 2022 5,137 1 View

I have a problem with calculating the CMC (critical micelle concentration) of beta casein using pyrene?

Hi The micellization of beta casein would occur in the condition described below, then we can calculate the CMC of protein by pyrene. The experiment conditions in my work were: 1.5 mg of pyrene...

18 February 2022 2,496 0 View

How should I do confocal microscopy imaging for drug treatment in LPS-induced endothelial cells?

I am working on inflammation in brain endothelial cells. I use LPS as inflammatory agent, then I treat my cells with a drug in order to reverse the LPS-related damage. I have three experimental...

17 February 2022 9,479 3 View

Badges
Science topic