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Questions related to Drugs
I'm currently analyzing the intestine using villin CRE knockout mice. While my team specializes in liver research and we're expanding into intestinal studies, we're not very familiar with...
19 April 2024 3,787 1 View
I tried glycine buffer but the yeast cells acidify the buffer so the pH goes down to 7-6 overnight. I need something that will stay around 9 and that isn't toxic to the cells.
19 April 2024 8,004 4 View
I directly cloned cry1A gene p234 expression vector and transformed E.coli Bl21 strain. but expression protein cannot appear.
19 April 2024 5,831 3 View
The non-linear response of the electron in the driving laser field, we always mention in solids, inter-band polarization, and coherent intra-band dynamics of the electron. I want to deepen my...
18 April 2024 3,422 1 View
i seeded CACO2 on 0,4 um PET transwell insert, i want to see my cell layer in transversal view in order to monitor the polarization and formation of brush border. To do this, i fixed with 4% PFA...
17 April 2024 6,530 1 View
microwave
17 April 2024 10,022 4 View
Due to factors outside of my control, peptides in my ESI-MS data have been ionised normally by protons ([M+2H], [M+3H]...) but also by sodium ([M+2Na], [M+3Na]...). Is there a way to configure...
17 April 2024 10,044 2 View
Example of data extraction from a study during a meta-analysis: A: reference. B: HR (95%CI) C: HR (95%CI) I am running a meta-analysis that combines B vs A+C. I could combine groups A+C using an...
16 April 2024 1,490 0 View
Dear all, I need to calculate the UV-Vis spectra with an explicit solvent. For that, I think I need to conduct some MD simulations, extract the structure from the MD results, and then perform...
16 April 2024 4,740 0 View
How to treat it
14 April 2024 6,892 1 View
During Analysis of residual solvents found An unknown peak so my question this which guideline to be used for investigation And what is limit of unknown peak in PPM And how is calculated
13 April 2024 9,523 2 View
I am using lammps real unit where the force is in kcal/mol-angstrom^3. So I need to convert my 1 bar pressure to Kcal/mol-angstrom^3 to get ta proper force to apply in the simulatio.I have...
13 April 2024 4,743 1 View
Before implementing low flow anesthesia, several prerequisites need to be considered to ensure patient safety and optimal delivery of anesthesia.
11 April 2024 499 1 View
Hello everyone, I am working on the production of IVT mRNA. After transcription, the remaining DNA templates are digested by DNase I. I have tested transcription with uncut DNA (mostly...
11 April 2024 1,369 2 View
Hi everyone, I'm a PhD student at CAST, Analytical Biochemistry and Proteomics Research Unit, University, "G. d'Annunzio" of Chieti-Pescara. We currently use the Thermo Fisher Orbitrap Fusion...
11 April 2024 9,359 0 View
Hello! I have tried to electroporate the Jurkat cell line with the Neon System using the parameters provided by the supplier, but almost all the cells die after the procedure. I have also tried...
11 April 2024 1,398 0 View
I am trying to isolate exosomes from the cell culture supernatant of Adipose Mesenchymal Stem Cells (20-30 ml) by the PEG isolation method. But exosome pellet is not visible after isolation. DLS...
09 April 2024 3,892 1 View
which is the most successful and guaranteed method, cause I Tried sodium fluoride 20mg/kg and it didn't work
09 April 2024 8,422 0 View
The answer is related to the definition of both food security and food safety
09 April 2024 7,213 8 View
Quite a naive question I am looking for the most optimal way to transfect different cell lines with the same construct. Some of them are notoriously difficult to transfect, like RWPE1 I am...
07 April 2024 2,310 3 View
What are the laboratory procedures for identifying the concentrations of formic acid and acetic acid in aqueous solutions using titration, spectrophotometry, or gas chromatography methods...
07 April 2024 195 3 View
This question is derived from the article Underfilling of vacuum blood collection tubes leads to increased lactate dehydrogenase activity in serum and heparin plasma samples.
07 April 2024 6,032 2 View
Hello everyone, I'm a master student and in my project I'm studying RFP-based constructs (RFP + few amino acid-long tags). I'm now performing a Western blot in SDS-PAGE of my cell-line lysates....
05 April 2024 7,397 3 View
Tl;dr - As of last week, the UV-Vis instrument at my job only records negative absorbance below 230 nm for any sample when zeroing methanol to correct for solvent. Attached spectra of the same...
04 April 2024 5,284 5 View