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i want to know why we are only doing double digestion in genomic DNA. also I want to know why we are doing both single digestion and double digestion in Plasmid DNA
19 August 2024 9,614 0 View
I am trying to calculate the recombination coefficients from the light-intensity-dependent Voc measurement. When I fitted the measured results following the equation Voc =...
19 August 2024 4,145 3 View
I am trying to inhibit NMD in HEK293T cells and reading different publications I am finding different protocols. Does anyone have experience whats the best combination of CHX concentration and...
19 August 2024 6,597 1 View
Hi, I recently did RNA seq for my samples previously having done qRTPCR for a select number of genes. However as I was analyzing the data I noticed a few discrepancies. In my sample for QRTPCR...
18 August 2024 2,112 5 View
I was performing an MTT assay to check cell viability. Due to a technical issue in the plate reader I was using, I had to read the plate at 540 nm wavelength. Would the results still be credible?
18 August 2024 6,393 5 View
I have fibrin from human plasma insoluble powder (Cat No. F5386-1G). I have to crosslink it with X compound but before crosslinking I have to make it soluble.
17 August 2024 9,197 1 View
Can I use a glass substrate rather than mica to characterize liposomes in AFM?
16 August 2024 9,467 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
12 August 2024 5,637 4 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
10 August 2024 10,017 2 View
The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.
08 August 2024 9,722 1 View
I have incorporated the dispersion effect for the OPBE functional by specifying the S6, SR6 and S8 parameters for GD3 in Gaussian G09 package. For this purpose, I have defined the environment...
06 August 2024 2,148 2 View
If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...
04 August 2024 5,980 2 View
I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...
04 August 2024 2,516 1 View
why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...
04 August 2024 4,518 3 View
How is matter moved through an ecosystem and movement of energy different from the movement of nutrients through ecosystems?
04 August 2024 5,390 0 View
content validity index
04 August 2024 2,362 0 View
I am looking for an expert on the flora of central and southern Italy and Sicily. I am offering co-authorship of a monograph on a historic herbarium kept in Poland. We have 300 high resolution...
02 August 2024 6,883 2 View
Research on politics administration relation can conduct based on case study method?
02 August 2024 9,671 1 View
How to identify the type of ions like cation or anion present in the electrolyte involving in charge storage mechanism for 3-electrode measurements?
02 August 2024 8,966 0 View
Solubility of Uric acid is very low in Saline or water, it needs to be prepared with a very high amount of NaOH to get to pH of around 13, but every article we found talked about preparing Uric...
01 August 2024 4,418 0 View
We are looking for projects for a team of professional developers About us We are a team of experienced developers with over 10 years of experience in various programming fields. Our mission is to...
31 July 2024 8,990 0 View
Hello everyone, As my title mentions when RASTtk is detecting Open Reading Frames in a genomes it uses a combination of both Glimmer3 and Prodigal, however I am a little confused in how the...
30 July 2024 5,569 0 View
I have different polymer solutions and I hypothesize that water molecules are retained based on the water-polymer interactions, so the amount of free water is less to intercat with drug tablet....
29 July 2024 10,215 6 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
28 July 2024 7,439 1 View