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We are trying to measure intracellular H2O2 in spheroids post exposure to nanozymes, that should generate ROS, using the ROS-Glo kit from Promega. However, we are getting strange results. The...
10 June 2024 2,740 0 View
How do I extract phospholipids from soy lecithin, and what maximum concentration can I get from soy lecithin?
08 June 2024 5,588 0 View
Comprehensive Review of Warfarin In what ways can healthcare professionals collaborate to optimize warfarin therapy and ensure patient safety and efficacy?
06 June 2024 4,919 1 View
PAC on OriginPro
05 June 2024 5,866 0 View
Hello Peers, Warm wishes to all. I am a new user of the Cytiva AKTA Start system for SEC with a column, HiPrep 16/60 Sephacryl S-100 HR (120 ml column volume). I am not sure about the minimum...
04 June 2024 7,705 2 View
I want to ask about the response of the analyte in the EIS measurement. Bare shows a high Rct response as compared to material-modified electrodes. But when we modify the material + analyte, the...
03 June 2024 860 3 View
I started a cell culture with a mix of suspended and adherent cells (presumably fibroblasts) on May 10th. After a week, I treated them for adherent cells only. On May 24th, most of the adherent...
03 June 2024 4,890 5 View
Hi all!...
30 May 2024 7,778 4 View
Hello! I am having trouble staining for plasma membrane localization of a transporter. This transporter also resides intracellularly, however, I am only interested in staining its plasma membrane...
29 May 2024 8,230 5 View
I want to determine if a particular monomer does not dissolve properly in my reaction solvent , N,N-diemthyl acetamide.
28 May 2024 626 3 View
i need very much thanks in advance
24 May 2024 5,000 2 View
How to purify boron esters? Hello. I'm synthesizing borates in my lab. My questions are as follows: 1. How to purify boronate esters like B(OR)3 compounds, and it is a UV undetected...
24 May 2024 7,061 2 View
Can data that includes less than values (below the limit of detection in chemical analysis) be used in ANOVA if the data is re-coded in some way? Perhaps using the actual detection limit or half...
24 May 2024 7,166 3 View
The desired gene region was amplified by PCR and the resulting PCR products were cleaned. Bands were observed in an agarose gel and measured in a spectrophotometer. Then, transcription was...
23 May 2024 6,790 1 View
Hi~ We are making different Cas9 plasmids such as regular spCas9, base editing and prime editing under different promoter such as CMV and CAG. During selection of colonies, we always get a good...
23 May 2024 7,321 0 View
I'm trying to synthesize a highly polar compound which is uv inactive (In TLC charring at the bottom- 0.1RF/20%MeOH:DCM ,MP) . I have confirmed the compound by simple mass and H'NMR spectrums but...
23 May 2024 9,809 3 View
I have 6 bacterial isolates, I have checked their band on agarose gel electrophoresis, I am getting a genomic band but when I am performing the PCR and after running the gel, I am not getting good...
22 May 2024 8,603 4 View
In this online file you have included my last 2 publications. But your original file my (Mohammad Ansaruzzaman, student of free university Brussels & Karolinska Institute) you have included...
22 May 2024 110 1 View
Hi, our lab has recently started working with primary hepatocytes in 3D form. The lot we are using has high basal activity which declines as the spheroids age. We are looking to undertake drug...
22 May 2024 1,308 0 View
I am confused regarding the desired edit efficiency of PEP2 for transversion on rice plants using Agrobacterium.
22 May 2024 4,306 0 View
I am looking for the optimized protocol for protoplast isolation by using just expanded unifoliate leaf of Mung Bean.
22 May 2024 3,514 1 View
I was hoping anyone could provide any insight into using ATCC's "RD-ES" and "A-673" cell lines. How many passages do you do before you notice any senescence or genetic abnormalities
21 May 2024 582 4 View
Suppose I use "pBABE-puro-GFP-wt-GeneX" to get GeneX expressing with GFP (I am not sure whether it will replace the endogenous GeneX or get inserted somewhere else since the pBABE-puro vector uses...
20 May 2024 4,707 0 View
How can i dilute my master stock of 1 micromolar DNA template to different concentration by serial dilution
18 May 2024 3,628 2 View