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Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
21 July 2024 4,140 2 View
Serotonin (5-hydroxytryptamine, 5-HT) is predominantly known as a neurotransmitter in the central nervous system (CNS), where it plays a crucial role in mood regulation, gastrointestinal motility,...
21 July 2024 3,920 5 View
Hi, everyone This figure depicts a simulation of an electrostatic energy harvesting system in COMSOL Multiphysics software. My question is regarding the relationship between the changes in...
20 July 2024 4,691 4 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
20 July 2024 2,153 0 View
The adsorbate is Arsenic and the adsorbent is a soil
19 July 2024 329 8 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
19 July 2024 9,173 2 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?
19 July 2024 9,830 0 View
Cuando se considera es el momento de jimar o cocechar la piña del agave para enviarla a destilación en la tequilera? con cuanto de fructanos y de azucares?
18 July 2024 683 0 View
How does leadership affect business intelligence adoption
18 July 2024 2,342 3 View
Hello everyone, I am cultivating patient derived organoids from pancreatic cancer patients and I saw at the bottom of my domes some "strange" cells other than my usual organoids. I know I should...
16 July 2024 759 3 View
I would like to do peptide map of a protein which is attached to PEG via lysines. Now the best approach would be to de-PEGylate it but then do the peptide map. But there is little literature on...
15 July 2024 5,632 2 View
Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...
15 July 2024 947 2 View
The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65...
15 July 2024 1,479 0 View
I am working on powder metallurgy, and during the compaction process, the powder does not pelletize and remains in its powder form. To address this issue, I am considering using binders to improve...
14 July 2024 9,001 2 View
TPA is a cornerstone in health insurance as every insurer after enrolling in a policy is waiting to get the benefit out of it, a human nature. because of existing norms, there is a gap between...
13 July 2024 7,468 0 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
13 July 2024 778 0 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
13 July 2024 6,311 0 View
Hello! Have you ever observed that when measuring the viability of freshly thawed PBMC with trypan blue, the value is higher than with Annexin/PI staining or some other staining that takes into...
11 July 2024 638 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
11 July 2024 4,914 2 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
11 July 2024 9,160 1 View
Hi, Does anyone have experience and success of isolating, culturing and stimulating hamster splenocytes for T-cell analysis (FACS and ELISPOT)? I am currently using a protocol that we routinely...
10 July 2024 6,334 1 View
Recently I looked up a literature《A Glucose-6-Phosphate Isomerase Peptide Induces T and BCell-Dependent Chronic Arthritis in C57BL/10 Mice Arthritis without Reactive Oxygen Species and...
10 July 2024 6,652 0 View
Lorsque nous nous posons cette question , nous nous basons sur un fait et constat très simple : Dans les manuels d’apprentissage du chinois, qui tiennent lieu de programme pour l’instant dans les...
09 July 2024 7,648 3 View
Hi, I am trying to model laser cladding in Ansys FLUENT. There are two phase: one is base metal surrounded by an inert gas (shown in the image). I want to add a cladding layer of the same metal....
08 July 2024 1,177 8 View