When Hematoxylin - Eosin staining is performed in mice tumors, which are the most important tumor tissue characteristics that determine the existence of necrotic zones? What changes in tumor tissue morphology occur when it is necrotic?
Many thanks for your answer! Much appreciated!
I am comparing the results of ex vivo BLI and H&E staining for mouse organs. Some organs appear to have tumors in BLI results but not in H&E or the opposite. Why this can happen?
06 February 2024 9,832 0 View
Dear ResearchGate Community, I am currently working on a research paper titled "A Metamathematical Approach to the Susceptible-Infected-Recovered (SIR) Model: The Case of the Hong Kong Flu in New...
05 August 2023 9,037 4 View
I have some flow cytometry issues with T-bet staining, thus I would like to ask about the transcription factor's approximate percentage (a mean, actually ) in peripheral blood samples from healthy...
08 October 2022 2,416 2 View
We are performing FISH in plasma cells. When it comes to back up, we are using a small amount of EDTA in order to store those cells. In case of a repeat, the EDTA creates a kind of membrane in the...
29 June 2021 8,989 1 View
I would like expert opinion on the following case: 84 yo male, with intermittent episodes of painless jaundice under investigation and known Hx of polycythemia rubra vera, under effective...
16 January 2021 1,449 6 View
In the context of visuspatial skill/sequential motor learning if that is of any help or relevance. Thank you in advance for any responses.
18 February 2020 1,887 0 View
I know it's not easy to find such a cell line (most of them are models of cancer afterall and it's also impossible for a cell line to produce autoantibodies), but I want to perform some in vitro...
10 January 2020 8,069 0 View
I use human blood samples (~8ml each) from healthy individuals and perform centrifuge over lymphoprep, in order to isolate the PBMCs. Then, I use the EasySep Kit to isolate the CD19+ cells. The...
17 November 2019 8,890 3 View
I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...
22 September 2019 3,210 3 View
thank you in advance!
11 August 2019 7,682 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
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I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View