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It is a feedback question for Reserach purpose.
03 April 2024 3,130 0 View
Public sectors need improvement in the way they manage their projects. most of the projects don't achieve its goals.
03 April 2024 9,794 5 View
Hello, Background: I am trying to find the Vmax and Km values for 0.25% trypsin and the substrate casein. During optimization, I have tried different time points (1-15 minutes) and different...
02 April 2024 1,546 1 View
Hi All, I have a following question: How much difference of isoelectric point between a wild type and mutant protein ca be accepted? E.g. The isoelectric points of my target wild type protein...
02 April 2024 1,841 0 View
Purpose of ECMO is to provide temporary support to failing respiratory and/or cardiac systems, allowing time for the underlying condition to improve or for more definitive treatments to be...
02 April 2024 1,800 0 View
Hello everyone ! I got turbidity 448 NTU in infrared light 860 nm ( ISO 7027 ) using a turbidimeter ( TB211 IR ). And it's not very clear to me exactly what formulas can be used to calculate...
30 March 2024 4,809 1 View
Dear colleagues, I am encountering an issue with our recently donated Sciex API 4000 LC-MS/MS system. As indicated in the attached data, I am not receiving any signal above 175 m/z. Has anyone...
30 March 2024 5,406 5 View
Hello, I have 200 chemical structures in a single ChemDraw file that I need to get 200 distinct files with single structure each. Can this be achieved, please?
29 March 2024 7,113 0 View
Hi all, I have been attempting to blot for LC3I and II and i have been having a strange issue, the bands seem to be showing up around 23 kDa. I have done the blot twice now and still the same...
29 March 2024 3,614 5 View
Hot air oven vs laboratory tray dryer, which is more suitable equipment for moisture analysis of food samples for short period?
28 March 2024 9,285 5 View
why multivariate test table shows non significant value whereas univariate shows significant value
28 March 2024 9,260 4 View
In my LC-MS experiment, I am utilizing an Agilent Polaris NH2 column to separate a mixture of glucose, fructose, and sucrose. I am using water and acetonitrile as A and B mobile phases...
27 March 2024 8,885 4 View
Lately, I’ve been encountering issues with the longevity of my Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) columns. Specifically, I use the ZORBAX Eclipse Plus C18 RRHD (Rapid...
27 March 2024 5,124 6 View
I did IHC using fluorescent labeled secondary antibodies to detect astrocytes with GFAP marker. My sample was fixed frozen mouse brain tissue streatum region. I saw fluorescence in my sample...
26 March 2024 2,585 3 View
Greetings esteemed researchers! I am relatively new to the field and would greatly appreciate some guidance on a challenge I'm encountering in my research. We're currently engaged in a process...
26 March 2024 4,580 4 View
What are the differences between 50 mM glycine-hydrochloric acid buffer (pH 3) and 100 mM glycine-hydrochloric acid buffer (pH 3), especially regarding osmotic pressure? Why do they have the same...
25 March 2024 8,896 1 View
I am finding out the relationship between culture shock and Exam scores of a group of participants. I have the culture shock level and marks both at the end of 1st semester and 2nd semester. How...
25 March 2024 281 1 View
An enzyme protein overexpressed in bacteria needs to be purified to show its activity. Why doesn't the cell lysate show activity when added to the substrate?
24 March 2024 9,441 9 View
I am conducting research on culture shock. My research question is: "Does culture shock affect the second language learning outcome (Marks) of Arab Chinese language learners in China? If so,...
24 March 2024 9,227 0 View
I have been using primary unconjugated TH antibody followed by a fluorescence tagged secondary antibody for the noradrenergic neurons of my brain stem sections. Due to some changes in my protocol,...
21 March 2024 2,615 2 View
Can biochar replace fertilizer and what happens to carbon in the soil after biochar is applied and effect of biochar on nitrogen use efficiency?
21 March 2024 4,879 0 View
Hi all. I am interested in the possibility of replacing the technology for producing tablets with the preliminary production of a solid dispersion by spray drying. My task is to develop a generic...
21 March 2024 4,947 1 View
I'm moddeling a steel-timber connection (single shear static test) using ea dynamic explict analysis in abaqus. Innitially i've tried to model the conenction using a static solver, but at some...
19 March 2024 5,965 4 View
Can anyone please suggest me some commercially available kits specifically designed for sequencing RNA viruses (in particular I am targeting a positive ssRNA virus that does not have a poly(A)...
19 March 2024 6,359 1 View