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I have modified the protein and I am simulating it using GROMACS. I have performed the 20 ns simulation after energy minimization and during visualization in VMD I observed that a few frames have...
13 February 2024 6,023 4 View
How much entropy is in the universe and what happens if the entropy of the universe is positive?
13 February 2024 9,125 2 View
I've been trying to figure out what happened but it seems I am the only one this has happened to? Processing: - transcardial perfusion with 1x PBS followed by 4% PFA - post-fix brain in 4% PFA...
13 February 2024 5,961 2 View
I would like to know the void fraction for known powder material properties to estimate a bisque sample's packing density/void fraction. In combination with any geometric &/or Archimedes...
13 February 2024 9,591 1 View
I am looking to remove divalent metal ions from growth medium like MHB. Is there any commercial materials available like resin, which can be separated easy by filtration? Your comments are...
12 February 2024 3,106 0 View
Hello everyone, does anyone know how to fit the letters inside the circles? I have tried everything but I haven't been able to... Thank you
11 February 2024 982 1 View
Requested to Discuss the challenges associated with the development and proliferation of AI-driven military capabilities, including the potential for destabilizing arms races and the need for...
11 February 2024 3,322 0 View
I have FTIR spectra and I need to calculate their FWHM. I tried many times on Origin, but never worked. I guess that is something related to the old version that I am using (8.5). Can you help...
10 February 2024 4,237 7 View
Is one of the aflatoxin (afl) genes mutated/not expressed? If so, which one?
10 February 2024 2,462 4 View
Hi to all, I am trying to dissolve green-synthesized metal oxide nanoparticles for DPPH and ABTS assays. I have tried 70% ethanol and DMSO, but no way. Can any one suggest other solvent(s)? N.B....
09 February 2024 8,355 3 View
Dear Scientists, Does Archives of Clinical and Medical Case Reports is predatory journal? [ISSN: 2575-9655] Impact Factor: 3.1 Indexing: PubMed / PMC Best regards Dr. Abdulla Al Mamun Bhuyan
09 February 2024 9,345 1 View
Hi, I'm planning to stimulate antigen specific T cells with CD3/CD28 microbeads for lentiviral transduction, and I'm also planning to stain TCRs after stimulation prior to TCR functional assays,...
08 February 2024 1,613 4 View
I would like to participate in this conference and would appreciate hearing from others about their experiences at previous conferences. It would also be helpful if any participants have written a...
08 February 2024 1,534 0 View
I am comparing the results of ex vivo BLI and H&E staining for mouse organs. Some organs appear to have tumors in BLI results but not in H&E or the opposite. Why this can happen?
07 February 2024 9,831 0 View
Hi everyone! I have a problem with slime in my protein sample. For isolation, I use RIPA buffer + protease inhibitors. When I measured the protein concentration immediately after isolation...
07 February 2024 4,797 1 View
I am in the process of setting up a method for determination nitroso diethanolamine in shampoo. This method is performed using derivatization and post-column with HPLC device and UV detector. I...
07 February 2024 9,040 9 View
Hi! I was wondering if anyone has taken confocal images of apoptotic cells stained with Apotracker Tetra, from Biolegend. There is information using Apotracker Green, but I haven´t found a...
07 February 2024 8,341 0 View
Dear researchers Please, I would like to know how to prepare the samples for the research and observation of viruses under the electron microscope? What is the best electron microscope to...
06 February 2024 4,927 2 View
Sustainable production of chemicals, pharmaceuticals, and clean water
03 February 2024 1,592 0 View
I have papers
02 February 2024 7,534 1 View
I am aware of the triple mutated version (K219A-D362A-D384A) of PINK1, however, I am looking to do a CRISPR into human neuronal cells and the simpler, the better the approach. I have seen that the...
31 January 2024 7,031 2 View
I am working in combinatorial approach in targeted cancer therapy. I have performed certain Insilco studies in screening of individual drug as protein target. However, I am wondering if it is...
31 January 2024 5,366 0 View
Hello everyone! I am considering the possibility of assisting Congress or Conferences on Drug Delivery Systems or Pharmaceutical Formulation. However, I do not have much experience in this...
31 January 2024 7,439 0 View
Every time I conduct protein crystallization experiments, it takes a considerable amount of time, often extending into the late hours of the night. Therefore, I am considering flash-freezing the...
30 January 2024 8,668 3 View